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Item Method of Using Carbonic Anhydrase to Detect Hemolysis(2011-05) Kline, Jeffrey A.; Zagorski, JohnA method and a test for using carbonic anhydrase (CA), particularly CA-I or CA-II, as a biomarker ofhemolysis. The method and test detect hemolysis by determining a percentage erythrocyte hemolysis in a specimen or sample of blood based upon quantification of carbonic anhydrase present in the extracellular portion of the blood. The method and test serve to optimize therapeutic efficacy for treatments of hemolysis. Plasma carbonic anhydrase is used to determine the percentage hemolysis in plasma. Furthermore, CA is quantified with specificity to the isozyme present in the plasma.