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Item The Ethylene Signaling Pathway Negatively Impacts CBF/DREB-Regulated Cold Response in Soybean (Glycine max)(Frontiers, 2019) Robison, Jennifer D.; Yamasaki, Yuji; Randall, Stephen K.; Biology, School of ScienceDuring cold stress, soybean CBF/DREB1 transcript levels increase rapidly; however, expected downstream targets appear unresponsive. Here we asked whether the ethylene signaling pathway, which is enhanced in the cold can negatively regulate the soybean CBF/DREB1 cold responsive pathway; thus contributing to the relatively poor cold tolerance of soybean. Inhibition of the ethylene signaling pathway resulted in a significant increase in GmDREB1A;1 and GmDREB1A;2 transcripts, while stimulation led to decreased GmDREB1A;1 and GmDREB1B;1 transcripts. A cold responsive reporter construct (AtRD29Aprom::GFP/GUS), as well as predicted downstream targets of soybean CBF/DREB1 [ Glyma.12g015100 (ADH), Glyma.14g212200 (ubiquitin ligase), Glyma.05g186700 (AP2), and Glyma.19g014600 (CYP)] were impacted by the modulation of the ethylene signaling pathway. Photosynthetic parameters were affected by ethylene pathway stimulation, but only at control temperatures. Freezing tolerance (as measured by electrolyte leakage), free proline, and MDA; in both acclimated and non-acclimated plants were increased by silver nitrate but not by other ethylene pathway inhibitors. This work provides evidence that the ethylene signaling pathway, possibly through the action of EIN3, transcriptionally inhibits the CBF/DREB1 pathway in soybean.Item LITHIUM-AQUEOUS BATTERY(Office of the Vice Chancellor for Research, 2012-04-13) Cavazos, Ana; Mosier, Luke; Chen, Rongrong; Kim, YoungsikDue to the exceptionally high energy density Lithium-water batteries have very high storage efficiency. Being able to store more energy is im-portant to many industries including electronics and electric vehicles. This is the reason that much research is being done to optimize and explore new techniques of development for these batteries. The Li-water battery has been designed in this project to test water and other aqueous solutions as the cathode. The lithium in a non-aqueous elec-trolyte acts as the anode of the battery. The solid electrolyte used in the lith-ium water batteries is a glass/ceramic (LISICON). The solid electrolyte acts as a separator allowing the Lithium ions to pass through it without allowing the liquid cathode come into direct contact the Lithium. This paper describes the creation and testing of a Lithium-water battery which uses water and Copper (II) Nitrate as the cathode electrolyte. The purpose of this paper is to compare and contrast the difference in voltage of distilled water and distilled water with Copper (II) Nitrate additives as cath-ode. When the tests were conducted, it was found that Copper (II) Nitrate does in fact increase the voltage of the Lithium-water batteries significantly when compared to the distilled water. These results were expected because of Copper (II) Nitrate’s strong electrolyte properties.