Browsing by Subject "adaptive optics"

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    Imaging and quantifying ganglion cells and other transparent neurons in the living human retina
    (National Academy of Sciences, 2017-11-28) Liu, Zhuolin; Kurokawa, Kazuhiro; Zhang, Furu; Lee, John J.; Miller, Donald T.; Engineering Technology, School of Engineering and Technology
    Ganglion cells are the primary building block of retinal neural circuitry, but have been elusive to observe and quantify in the living human eye. Here, we show a light microscopy modality that reveals not only the somas of these cells, but also their 3D packing geometry, primary subtypes, and spatial projection to other neurons. The method provides a glimpse of the rich tapestry of neurons, glia, and blood vessels that compose the retina, thus exposing the anatomical substrate for neural processing of visual information. Clinically, high-resolution images of retinal neurons in living eyes hold promise for improved diagnosis and assessing treatment of ganglion cell and other neuron loss in retinal disease., Ganglion cells (GCs) are fundamental to retinal neural circuitry, processing photoreceptor signals for transmission to the brain via their axons. However, much remains unknown about their role in vision and their vulnerability to disease leading to blindness. A major bottleneck has been our inability to observe GCs and their degeneration in the living human eye. Despite two decades of development of optical technologies to image cells in the living human retina, GCs remain elusive due to their high optical translucency. Failure of conventional imaging—using predominately singly scattered light—to reveal GCs has led to a focus on multiply-scattered, fluorescence, two-photon, and phase imaging techniques to enhance GC contrast. Here, we show that singly scattered light actually carries substantial information that reveals GC somas, axons, and other retinal neurons and permits their quantitative analysis. We perform morphometry on GC layer somas, including projection of GCs onto photoreceptors and identification of the primary GC subtypes, even beneath nerve fibers. We obtained singly scattered images by: (i) marrying adaptive optics to optical coherence tomography to avoid optical blurring of the eye; (ii) performing 3D subcellular image registration to avoid motion blur; and (iii) using organelle motility inside somas as an intrinsic contrast agent. Moreover, through-focus imaging offers the potential to spatially map individual GCs to underlying amacrine, bipolar, horizontal, photoreceptor, and retinal pigment epithelium cells, thus exposing the anatomical substrate for neural processing of visual information. This imaging modality is also a tool for improving clinical diagnosis and assessing treatment of retinal disease.
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    Secret key distribution leveraging color shift over visible light channel
    (IEEE, 2017-10) Liu, Hongbo; Liu, Bo; Shi, Cong; Chen, Yingying; Computer and Information Science, School of Science
    Given the widely adoption of screen and camera in many electronic devices, the visible light communication (VLC) over screen-to-camera channel emerges as a novel short range communication technique in recent years. Active research explores various ways to convey messages over screen-camera channel, such as barcode and unobtrusive optical pattern. However, with the prevalence of LED screens of wide viewing angles and mobile devices equipped with high standard cameras, the threat of information leakage over screen-to-camera channel becomes in-negligible. Few studies have discussed how to ensure the security of data transmission over screen-to-camera channel. In this paper, we propose a secret key distribution system leveraging the unique color shift property over visible light channel. To facilitate such design, we develop a practical secret key matching based method to map the secret key into gridded optical patterns on screen, which can only be correctly recognized by the legitimate user through an accessible region and allow regular data stream transmission through valid grids. The proposed system is prototyped with off-the-shelf devices and validated under various experimental scenarios. The results show that our system can achieve high bit-decoding accuracy for the legitimate users while maintaining comparable data throughput as regular unobtrusive VLC systems with very low recovery accuracy of the encrypted data for the attackers.