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Item Fluoride uptake by enamel from stannous fluoride and prophylaxis pastes(1975) Sokoloff, Philip, 1947-The purposes of this study were twofold: (1) to determine whether applying zirconium silicate prophylaxis paste (containing nine per cent stannous fluoride) prior to topical application of 10 per cent aqueous solution of stannous fluoride would promote a significantly greater enamel fluoride uptake than when the solution was applied alone; and (2) to determine whether rinsing the mouth with tap water immediately after these procedures would significantly affect enamel fluoride uptake. A modification of an in vivo, acid-etched enamel biopsy technique was used to measure fluoride and calcium contents of the outermost layer of enamel in first permanent molars and primary cuspids of 89 children. Four sites were biopsied prior to treatment. Treatments involved prophylaxes with a zirconium silicate paste either containing or not containing nine per cent stannous fluoride, followed by either a four-minute topical application of 10 per cent stannous fluoride in an aqueous solution or no such topical application. Some participants were instructed not to rinse for one-half hour; others were immediately offered unlimited quantities of tap water. Four other sites were biopsied one week after treatment; four more were biopsied two weeks after treatment. Pre- versus post-treatment differences were measured via a fluoride-sensitive electrode and atomic absorption spectrophotometry. Results were inconclusive. Enamel fluoride uptakes for most treatment groups appeared to be negative. Due to this seeming disagreement with the findings of many other investigators, and due to the very small numbers within treatment groups and biopsy sites, it was felt that uptake comparisons from group to group would be misleading. Tentative explanations for the unexpected results were offered.Item The Impact of Mouthrinses on the Efficacy of Fluoride Dentifrices in Preventing Enamel and Dentin Erosion/ Abrasion(2018) Albeshir, Ebtehal; Lippert, Frank; Cook, Norman B.; Hara, AndersonObjective: Toothbrushing with fluoride toothpaste followed by rinsing with mouthwash is a routine procedure to maintain good oral hygiene. It is unknown to what extent these rinses can modulate the effect of fluoride in its ability to prevent erosion/abrasion.The aim of this in-vitro study was to investigate and compare the impact of chlorhexidine (CHX), essential oils (EO) and cetylpyridinium chloride (CPC) mouthrinses on erosive tooth wear protection afforded by conventional fluoride toothpastes. Materials and Methods: The following experimental factors were considered: five rinses: CHX, EO, CPC, a fluoride rinse, and deionized water, two fluoride toothpastes: stannous fluoride (SnF2) or sodium fluoride (NaF) and two models: (erosion/ erosion+abrasion). Slabs of bovine enamel and dentin were prepared and embedded in resin blocks and generated 10 enamel and dentin testing groups (n = 8). UPVC tapes were placed on the sides of each slab leaving 1mm area exposed in the center. The blocks were subjected to a five-day cycling model. Then, the blocks were placed in a brushing machine and exposed to fluoride toothpaste slurry (one side was brushed and the other wasn’t). The blocks were then exposed to rinse treatments. Artificial saliva was used to remineralize the specimens after erosions and treatment challenges, and as storage media. After the fifth day of cycling, surface loss (in micrometers) was determined by profilometer. Data were analyzed using ANOVA (α = 0.05). Results: There was no interaction among the three factors (type of toothpaste, mouthrinse and abrasion or not (dentin p = 0.0520, enamel p = 0.4720). There were no significant two-way interactions as SL was only affected by toothpaste and mouthrinse. NaF caused less SL than SnF2 (4.60 vs. 5.83 μm; p < 0.0001) in dentin, whereas the opposite was found in enamel (5.20 vs. 3.56 μm; p < 0.0001). Toothbrushing abrasion caused comparatively more SL in enamel (6.53 vs. 2.23 μm; p < 0.0001) than in dentin (6.06 vs. 4.38 μm; p < 0.0001). None of the tested mouthrinses affected SL. Conclusion: Commonly used mouthrinses containing antimicrobial agents or additional fluoride, do not impair the erosion/abrasion protection afforded by fluoride toothpastes. Tested SnF2 dentifrice offered greater protection against enamel surface loss and NaF dentifrices showed more protection for the dentin surface. Clinical relevance: The understanding of the interaction between commonly used rinses and fluoride dentifrices will help dentists provide better recommendations to patients with erosive lesions.Item Influence of dentrifice abrasivity and toothbrush stiffness on the development of non-carious cervical lesions(2016) Binsaleh, Fahad; Hara, Anderson T.; Lippert, Frank; Bottino, Marco; Cook, Norman Blaine; Diefenderfer, Kim EdwardBackground: Non-carious cervical lesions (NCCLs) can be defined as the loss of dental hard tissue near the cemento-enamel junction without bacterial involvement. Abrasion, erosion and abfraction have been mentioned as common etiological factors of NCCLs. Abrasion is the loss of tooth structure due to friction by materials such as toothbrushes or abrasives in toothpaste. In contrast, dental erosion is the loss of tooth structure driven by acids. Abfraction, on the other hand, starts due to the weakening of the tooth structure in areas of concentrated stress as a result of cuspal flexure from heavy and repeated occlusal loading, which progresses to dental hard-tissue loss. Purpose: The present study focused on the abrasion aspect of NCCLs. Specifically, it aimed to investigate the influence of dentifrice abrasivity and toothbrush stiffness on the development of NCCLs in vitro Hypothesis: NCCL development is affected by both the abrasive level of the dentifrice and the stiffness of the toothbrush, as well as their interaction. Materials and Methods: A total of 288 extracted human upper first premolars, free of any dental caries and root defects, were selected. The teeth were be cleaned with a hand periodontal scaler and randomly assigned into twelve groups (total of 24 teeth/group). Specimens were brushed in an automated toothbrushing machine, using simulated toothpaste slurries of varying abrasivity and toothbrushes of varying stiffness. This study examined three experimental factors: 1. Toothpaste abrasivity, at four levels: high, medium, low, and non-abrasive slurry (as negative control); 2. Toothbrush stiffness, at three levels: soft, medium, and hard; 3. Toothbrushing cycles at three levels: baseline, 35k, and 65k strokes. Specimens were analyzed by optical profilometry at baseline and after each brushing level. The response variable was the dentin volumetric loss, in mm3. All toothbrushes caused significantly higher tooth wear when associated to the high abrasive slurry, compared to medium- and low-abrasive slurries. Medium- caused more tooth wear than low-abrasive slurry, which in turn led to more tooth wear than the control. Hard and medium toothbrushes were not significantly different, but both caused significantly higher volumetric loss than Soft toothbrushes. There were no differences among toothbrushes, when used with the non-abrasive (control) and low- abrasive slurries. Overall, 35k strokes resulted in significantly less tooth volumetric loss than 65k.Item Remineralizing Effect of Xilitol, Juniperus Communis and Camellia Sinensis Added to a Toothpaste: An In Vitro Study(University of Costa Rica, 2018) Jiménez-Gayosso, Sandra I.; Lara-Carrillo, Edith; Scougall-Vilchis, Rogelio J.; Morales-Luckie, Raúl A.; Medina Solís, Carlo E.; Velázquez-Enríquez, Ulises; Maupomé, Gerardo; Herrera-Serna, BrendaThe aim of this study was to explore the remineralizing effect of toothpastes based on Xilitol, Camellia Sinensis and Juniperus Communis. An in vitro experimental study was carried out in an 18 human premolars sample, which were treated with one of the 3 evaluated toothpastes and a control fluoride one. The atomic percentages of Ca and P were evaluated by Energy Dispersive X-ray Spectroscopy (EDS). In addition, the enamel surface of treated teeth was visualized by Scanning Electron Microscopy (SEM). The evaluations were carried out in three stages: pre-treatment, after an artificial demineralization process and after the treatment with the toothpastes. In the statistical analysis, the one-way ANOVA and Pearson correlation tests were used. Xilitol and Juniperus Communis showed the greatest gain of P ions compared to the control group (p‹0.01). As for Ca, the group treated with xylitol-based toothpaste showed more ion gain compared with the control group (p‹0.01). In the Pearson correlation test between Ca and P, statistically significant correlations were observed in all groups (p‹0.01), ranging between r=0.7413 (Xylitol Group) and r=0.9510 (Control Group). We concluded that Xylitol paste showed the highest remineralizing property, both in the EDS analysis and in the SEM images.