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Item Effect of dentifrice slurry abrasivity and erosive challenge on simulated non-carious cervical lesions development in vitro(J-STAGE, 2021-04) Nassar, Hani M.; Hara, Anderson T.; Cariology, Operative Dentistry and Dental Public Health, School of DentistryPURPOSE: To investigate the effect of slurry abrasive levels and acidic challenges on the development of non-carious cervical lesions. METHODS: Ninety-six extracted upper premolars were affixed in pairs to acrylic blocks and had their root surfaces covered by acrylic resin except for 2 mm from the cemento-enamel junction. The specimens were distributed into six groups (n = 8 pairs) based on two experimental factors: (1) slurry abrasivity level [low/medium/high] and (2) citric acid challenge [yes/no]. Specimens were brushed for 5,000, 15,000, 35,000, and 65,000 strokes. Volume loss (VL) was determined based on optical profilometry scans of specimens impressions at the baseline and at subsequent brushing levels. Data were analyzed using repeated measures analysis of variance and Bonferroni pairwise comparison (α = 0.05). RESULTS: Higher VL values were associated with high-abrasivity slurries relative to low- and medium-abrasivity slurries (P < 0.001). Increasing the slurry abrasivity level increased the VL regardless of the acidic challenge, which did not have a significant effect (P = 0.184). After 65,000 strokes, significant VL was recorded in all groups relative to preceding brushing levels (P < 0.001). CONCLUSION: Higher values of time-dependent surface loss were associated with increased dentifrice slurry abrasivity, regardless of the citric acid challenge.Item Effect of Toothbrushing on a Monolithic Dental Zirconia Submitted to an Accelerated Hydrothermal Aging(2022) Almajed, Norah; Sochacki, Sabrina Feitosa; Cook, Norman Blaine; Capin, Oriana ReisItem Fluoride Release from Two High-Viscosity Glass Ionomers after Exposure to Fluoride Slurry and Varnish(MDPI, 2019-11-15) Nassar, Hani M.; Platt, Jeffrey A.; Biomedical Sciences and Comprehensive Care, School of DentistryThe effect of brushing with different fluoride slurries on the fluoride release (FR) of different high-viscosity glass ionomer cements (GICs) was investigated. Fifty-eight discs were fabricated from two high-viscosity GICs (GC Fuji IX (F9) and 3M ESPE Ketac-fil (KF)). Five specimens from each brand were used to measure Vickers microhardness and the remaining were randomly assigned to one of four groups (n = 6) based on two-factor combinations: (1) fluoride concentration in the abrasive slurry (275 or 1250 ppm fluoride as NaF) and (2) immersion in a 22,500 ppm fluoride-containing solution. Specimens were brushed for a total of 20,000 strokes over 4 days with daily FR measurement. Data were analyzed using analysis of variance and Bonferroni tests (α = 0.05). Baseline FR and microhardness values were different between the two tested material brands. Exposure to a 22,500 ppm solution was associated with higher FR but not the exposure to 1250 ppm slurries. Brushing and immersion of glass ionomer cements in a 22,500 ppm F solution led to higher FR that was more sustained for KF. Type of the glass ionomer, progressive brushing, and fluoride varnish affected FR but not the fluoride content in the abrasive slurry.Item Influence of dentrifice abrasivity and toothbrush stiffness on the development of non-carious cervical lesions(2016) Binsaleh, Fahad; Hara, Anderson T.; Lippert, Frank; Bottino, Marco; Cook, Norman Blaine; Diefenderfer, Kim EdwardBackground: Non-carious cervical lesions (NCCLs) can be defined as the loss of dental hard tissue near the cemento-enamel junction without bacterial involvement. Abrasion, erosion and abfraction have been mentioned as common etiological factors of NCCLs. Abrasion is the loss of tooth structure due to friction by materials such as toothbrushes or abrasives in toothpaste. In contrast, dental erosion is the loss of tooth structure driven by acids. Abfraction, on the other hand, starts due to the weakening of the tooth structure in areas of concentrated stress as a result of cuspal flexure from heavy and repeated occlusal loading, which progresses to dental hard-tissue loss. Purpose: The present study focused on the abrasion aspect of NCCLs. Specifically, it aimed to investigate the influence of dentifrice abrasivity and toothbrush stiffness on the development of NCCLs in vitro Hypothesis: NCCL development is affected by both the abrasive level of the dentifrice and the stiffness of the toothbrush, as well as their interaction. Materials and Methods: A total of 288 extracted human upper first premolars, free of any dental caries and root defects, were selected. The teeth were be cleaned with a hand periodontal scaler and randomly assigned into twelve groups (total of 24 teeth/group). Specimens were brushed in an automated toothbrushing machine, using simulated toothpaste slurries of varying abrasivity and toothbrushes of varying stiffness. This study examined three experimental factors: 1. Toothpaste abrasivity, at four levels: high, medium, low, and non-abrasive slurry (as negative control); 2. Toothbrush stiffness, at three levels: soft, medium, and hard; 3. Toothbrushing cycles at three levels: baseline, 35k, and 65k strokes. Specimens were analyzed by optical profilometry at baseline and after each brushing level. The response variable was the dentin volumetric loss, in mm3. All toothbrushes caused significantly higher tooth wear when associated to the high abrasive slurry, compared to medium- and low-abrasive slurries. Medium- caused more tooth wear than low-abrasive slurry, which in turn led to more tooth wear than the control. Hard and medium toothbrushes were not significantly different, but both caused significantly higher volumetric loss than Soft toothbrushes. There were no differences among toothbrushes, when used with the non-abrasive (control) and low- abrasive slurries. Overall, 35k strokes resulted in significantly less tooth volumetric loss than 65k.Item Interaction between toothpaste abrasivity and toothbrush filament stiffness on the development of erosive-abrasive lesions(2015) Arrageg, Mona; Chu, Tien-Mien; Kelton, Stewart; Cook, Norman; Lippert, Frank; Hara, Anderson T.Background: Toothpaste abrasivity is considered the major contributor in toothbrushing abrasive wear, while toothbrush stiffness can be considered a secondary factor that may modify the abrasivity of toothpaste. Objectives: To investigate the longitudinal enamel and dentin surface loss caused by the interaction between the abrasives in toothpaste and toothbrush filament stiffness. Study Hypothesis: The amount of enamel and dentin loss depends on the abrasivity of the toothpaste and the filament stiffness of toothbrush. Materials and Methods: The following experimental factors were considered: abrasive suspension, at two levels (L-low: Z113 and H-high: Z103); and toothbrushes at three levels determined by bristle stiffness (soft, medium, and hard) generating 6 testing groups (n = 8). Slabs of bovine enamel and dentin were cut, embedded in acrylic resin, and polished. UPVC tapes were placed on the surface of the specimens, leaving an area of 1 × 4 mm exposed in the center of the each enamel slab. Specimens (n = 8) were subjected to 5 d of erosion/abrasion cycling: erosion (5min, 4×/d, 0.3% citric acid, pH 3.75), abrasion (15 s, 2×/d, 45 strokes each, 150-g load, automated brushing machine), fluoride treatment (15 s with abrasion and 45 s without abrasion; 275 ppm F as NaF in abrasive slurry) with exposure to artificial saliva between erosion and abrasion (1h) and all other times (overnight). Surface loss (SL, in micrometers) was determined by optical profilometry, after the third and fifth days of cycling. Data were analyzed using three-way ANOVA (alpha = 0.05). For enamel, only cycling time was found to affect surface loss with 5 d > 3 d. Overall, there was little SL (mean range: 0.76 µm to 1.85 µm). For dentin (mean SL range: 1.87 µm to 5.91 µm), significantly higher SL was found for 5 d vs. 3 d, with particularly large differences for hard toothbrush high abrasive, and medium toothbrush/low abrasive. Hard toothbrush resulted in significantly higher SL than medium toothbrush for high abrasive after 5 d, with no other significant stiffness differences. High abrasive had significantly higher SL than low abrasive overall with strong effects for all combinations, except medium stiffness after 5 d. In conclusion, the interplay between abrasivity and filament stiffness appears to be more relevant for dentin than enamel.