Browsing by Subject "Tetracycline"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    An evaluation of tetracycline stain removal by bleaching vital rabbit incisors
    (1974) Fleege, Patrick A.; Roche, James R., 1924-; Klein, Arthur Irving, 1922-2004; Katz, Simon, 1920-1987; Koerber, Leonard G.; Mitchell, David F.
    This study evaluated the effectiveness of bleaching tetracycline-stained teeth by measuring the loss of fluorescent intensity from teeth that were bleached. Nineteen male New Zealand white rabbits, with 58 incisors stained with oxytetracycline and 16 incisors as unstained controls, were used. Three rabbits were sacrificed to determine whether the tetracycline stain was comparable between incisors in the same jaw. Of the remaining 16 animals, 6 were bleached once and 10 were bleached twice. One maxillary and one mandibular incisor were bleached in each jaw with 30 percent hydrogen peroxide and heat for ten minutes per tooth; the other incisors were protected with a rubber dam. The animals were sacrificed 24 hours after the last bleach. The fluorescent intensity of 374 select ground sections 100 ± 5 microns thick from the incisal, middle and gingival thirds of the teeth were measured with an ultraviolet light microscope coupled to a television electronic measurement system. These measurements were statistically analyzed by t-test, and observations correlated. The dentin of tetracycline-stained maxillary incisors which were bleached twice and the dentin in the incisal one-third of the mandibular incisors which were bleached twice had a significantly (P ≤ 0.001, P ≤ 0.005) lower tetracycline fluorescent intensity than the dentin of unbleached tetracycline-stained teeth. The greatest loss of fluorescent intensity of tetracycline occurred in dentin closest to the dentino-enamel junction and varied from about 150 to 350 microns from the outer enamel surface. Clinical Kodachromes indicate that the loss of tetracycline pigment is associated with the loss of tetracycline fluorescence; The ground sections showed that the tetracycline fluorescence was never totally removed by two bleaches.
  • Thumbnail Image
    Item
    Novel bioactive tetracycline-containing electrospun polymer fibers as a potential antibacterial dental implant coating
    (SpringerLink, 2017-07) Shahi, R. G.; Albuquerque, M. T. P.; Münchow, E. A.; Blanchard, S. B.; Gregory, R. L.; Bottino, M. C.; Biomedical Sciences and Comprehensive Care, School of Dentistry
    The purpose of this investigation was to determine the ability of tetracycline-containing fibers to inhibit biofilm formation of peri-implantitis-associated pathogens [i.e., Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), Prevotella intermedia (Pi), and Aggregatibacter actinomycetemcomitans (Aa)]. Tetracycline hydrochloride (TCH) was added to a poly(DL-lactide) [PLA], poly(ε-caprolactone) [PCL], and gelatin [GEL] polymer blend solution at distinct concentrations to obtain the following fibers: PLA:PCL/GEL (TCH-free, control), PLA:PCL/GEL + 5 % TCH, PLA:PCL/GEL + 10 % TCH, and PLA:PCL/GEL + 25 % TCH. The inhibitory effect of TCH-containing fibers on biofilm formation was assessed by colony-forming units (CFU/mL). Qualitative analysis of biofilm inhibition was done via scanning electron microscopy (SEM). Statistical significance was reported at p < 0.05. Complete inhibition of biofilm formation on the fibers was observed in groups containing TCH at 10 and 25 wt%. Fibers containing TCH at 5 wt% demonstrated complete inhibition of Aa biofilm. Even though a marked reduction in CFU/mL was observed with an increase in TCH concentration, Pi proved to be the most resilient microorganism. SEM images revealed the absence of or a notable decrease in bacterial biofilm on the TCH-containing nanofibers. Collectively, our data suggest that tetracycline-containing fibers hold great potential as an antibacterial dental implant coating.
  • Thumbnail Image
    Item
    Tetracycline-incorporated polymer nanofibers as a potential dental implant surface modifier
    (Wiley, 2017-10) Bottino, Marco C.; Münchow, Eliseau A.; Albuquerque, Maria T.P.; Kamocki, Krzysztof; Shahi, Rana; Gregory, Richard L.; Chu, Tien-Min G.; Pankajakshan, Divya; Biomedical Sciences and Comprehensive Care, School of Dentistry
    This study investigated the antimicrobial and osteogenic properties of titanium (Ti) disks superficially modified with tetracycline (TCH)-incorporated polymer nanofibers. The experiments were carried out in two phases. The first phase dealt with the synthesis and characterization (i.e., morphology, mechanical strength, drug release, antimicrobial activity, and cytocompatibility) of TCH-incorporated fibers. The second phase was dedicated to evaluating both the antimicrobial and murine-derived osteoprecursor cell (MC3T3-E1) response of Ti-modified with TCH-incorporated fibers. TCH was successfully incorporated into the submicron-sized and cytocompatible fibers. All TCH-incorporated mats presented significant antimicrobial activity against periodontal pathogens. The antimicrobial potential of the TCH-incorporated fibers-modified Ti was influenced by both the TCH concentration and bacteria tested. At days 5 and 7, a significant increase in MC3T3-E1 cell number was observed for TCH-incorporated nanofibers-modified Ti disks when compared to that of TCH-free nanofibers-modified Ti-disks and bare Ti. A significant increase in alkaline phosphatase (ALP) levels on the Ti disks modified with TCH-incorporated nanofiber on days 7 and 14 was seen, suggesting that the proposed surface promotes early osteogenic differentiation. Collectively, the data suggest that TCH-incorporated nanofibers could function as an antimicrobial surface modifier and osteogenic inducer for Ti dental implants.