Browsing by Subject "Teeth"

Now showing 1 - 4 of 4
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    Differential profiles of soluble and cellular toll like receptor (TLR)-2 and 4 in chronic periodontitis
    (PLOS, 2018-12-20) AlQallaf, Hawra; Hamada, Yusuke; Blanchard, Steven; Shin, Daniel; Gregory, Richard; Srinivasan, Mythily; Periodontology, School of Dentistry
    Chronic periodontitis is a common inflammatory disease initiated by a complex microbial biofilm and mediated by the host response causing destruction of the supporting tissues of the teeth. Host recognition of pathogens is mediated by toll-like receptors (TLRs) that bind conserved molecular patterns shared by large groups of microorganisms. The oral epithelial cells respond to most periodontopathic bacteria via TLR-2 and TLR-4. In addition to the membrane-associated receptors, soluble forms of TLR-2 (sTLR-2) and TLR-4 (sTLR-4) have been identified and are thought to play a regulatory role by binding microbial ligands. sTLR-2 has been shown to arise from ectodomain shedding of the extracellular domain of the membrane receptor and sTLR-4 is thought to be an alternate spliced form. Many studies have previously reported the presence of elevated numbers of viable exfoliated epithelial cells in the saliva of patients with chronic periodontitis. The objective of this study was to investigate the potential value of salivary sTLR-2 and sTLR-4 together with the paired epithelial cell-associated TLR-2/4 mRNA as diagnostic markers for chronic periodontitis. Unstimulated whole saliva was collected after obtaining informed consent from 40 individuals with either periodontitis or gingivitis. The sTLR-2 and sTLR4 in saliva was measured by enzyme-linked immunosorbent assay. The TLR-2 and TLR-4 transcript in the epithelial cells in saliva was measured by real time polymerase chain reaction. While levels of sTLR-2 exhibited an inverse correlation, sTLR-4 positively correlated with clinical parameters in the gingivitis cohort. Interestingly, both correlations were lost in the periodontitis cohort indicating a dysregulated host response. On the other hand, while the sTLR-2 and the paired epithelial cell associated TLR-2 mRNA exhibited a direct correlation (r2 = 0.62), that of sTLR4 and TLR-4 mRNA exhibited an inverse correlation (r2 = 0.53) in the periodontitis cohort. Collectively, assessments of salivary sTLR2 and sTLR4 together with the respective transcripts in the epithelial cells could provide clinically relevant markers of disease progression from gingivitis to periodontitis.
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    EVALUATION OF MICROTENSILE BOND STRENGTH AND MICROLEAKAGE OF A ONE-STEP SELF-ETCH ADHESIVE
    (Office of the Vice Chancellor for Research, 2012-04-13) AlZain, Afnan; Eckert, George; Platt, Jeffrey
    New dental glue (G-aenial Bond-GB) was developed to increase the strength of bonding the white tooth filling to the tooth. An extra roughening step of the tooth surface using a specific acid should help the flow of the glue to the tooth and increases the strength of the bond between the tooth and the filling. Therefore, this study was done to evaluate how strong the bond is between the filling and the tooth using GB, and how much GB leaks and compare both tests with two other glue products and, with and without adding an extra roughening step. For the bond strength test, human molars teeth were divided into 5 groups, each containing 15 teeth. In 3 groups, each glue type was applied on tooth dentin according to company's instructions. In the last 2 groups an extra roughening step using a specific acid was added before applying the glue. The samples were stored in fake saliva where four samples were soaked for 48h and four samples were placed in a machine (thermocycling) that resembles drinking hot and cold beverages where samples are exposed to hot and cold water for 40 days and then the strength of the bond was tested (α=0.05). The broken edges were examined using a light microscope. For the leak test, human molars were divided randomly into the same 5 groups as bond strength test but each containing 11 teeth. A cavity was pre-pared and filled on the check and tongue sides of the tooth. Teeth were then also subjected to thermocylcling and stored for four weeks, soaked in dye for 24 hours and sectioned. The dye penetration was evaluated using light mi-croscopy (α=0.05). Bond strength of GB was significantly higher when an acid roughening step was added. No significant difference in leak of GB was observed.
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    Peri-Implant Bone Loss and Peri-Implantitis: A Report of Three Cases and Review of the Literature
    (Hindawi Publishing Corporation, 2016) John, Vanchit; Shin, Daniel; Marlow, Allison; Hamada, Yusuke; Department of Periodontics & Allied Dental Programs
    Dental implant supported restorations have been added substantially to the clinical treatment options presented to patients. However, complications with these treatment options also arise due to improper patient selection and inadequate treatment planning combined with poor follow-up care. The complications related to the presence of inflammation include perimucositis, peri-implant bone loss, and peri-implantitis. Prevalence rates of these complications have been reported to be as high as 56%. Treatment options that have been reported include nonsurgical therapy, the use of locally delivered and systemically delivered antibiotics, and surgical protocols aimed at regenerating the lost bone and soft tissue around the implants. The aim of this article is to report on three cases and review some of the treatment options used in their management.
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    Treatment of an Erratic Extraction Socket for Implant Therapy in a Patient with Chronic Periodontitis
    (Hindawi Publishing Corporation, 2016) Hamada, Yusuke; Prabhu, Srividya; John, Vanchit; Department of Periodontics & Allied Dental Programs
    As implant therapy becomes more commonplace in daily practice, preservation and preparation of edentulous sites are key. Many times, however, implant therapy may not be considered at the time of tooth extraction and additional measures are not taken to conserve the edentulous site. While the healing process in extraction sockets has been well investigated and bone fill can be expected, there are cases where even when clinicians perform thorough debridement of the sockets, connective tissue infiltration into the socket can occur. This phenomenon, known as "erratic healing," may be associated with factors that lead to peri-implant disease and should be appropriately managed and treated prior to surgical implant placement. This case report describes the successful management of an erratic healing extraction socket in a 62-year-old Caucasian male patient with chronic periodontitis and the outcomes of an evidence-based treatment protocol performed prior to implant therapy. Careful preoperative analysis and cone beam computed tomography imaging can help detect signs of impaired healing in future implant sites and prevent surgical complications.