Browsing by Subject "TCPTP"

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    TCPTP-deficiency in muscle does not alter insulin signalling and glucose homeostasis.
    (Springer, 2012-02) Loh, Kim; Merry, Troy L.; Galic, Sandra; Wu, Ben J.; Watt, Matthew J.; Zhang, Sheng; Zhang, Zhong-Yin; Neel, Benjamin G.; Tiganis, Tony; Department of Biochemistry and Molecular Biology, IU School of Medicine
    Aims/Hypothesis: Insulin activates the insulin receptor (IR) protein tyrosine kinase and downstream phosphatidylinositol-3-kinase (PI3K)/Akt signalling in muscle to promote glucose uptake. The IR can serve as a substrate for the protein tyrosine phosphatases (PTP) 1B and TCPTP, which share a striking 74% sequence identity in their catalytic domains. PTP1B is a validated therapeutic target for the alleviation of insulin resistance in type 2 diabetes. PTP1B dephosphorylates the IR in liver and muscle to regulate glucose homeostasis, whereas TCPTP regulates IR signalling and gluconeogenesis in the liver. In this study we have assessed for the first time the role of TCPTP in the regulation of IR signalling in muscle. Methods: We generated muscle-specific TCPTP-deficient (MCK-Cre;Ptpn2lox/lox) mice and assessed the impact on glucose homeostasis and muscle IR signalling in chow versus high fat fed mice. Results: Blood glucose and insulin levels, insulin and glucose tolerances and insulininduced muscle IR activation and downstream PI3K/Akt signalling remained unaltered in chow fed