Browsing by Subject "Rabbits"
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Item Analysis of Bone Remodeling in the Mandibular Condyle of Female Retired-Breeder Rabbits Following Altered Loading(1992) Hunt, J. Todd; Garetto, Lawrence P.; Roberts, W. Eugene; Arbuckle, Gordon R.; Brown, David T.; Shanks, James C.Previous studies have associated increased occlusal loads with degenerative changes of the temporomandibular joint (TMJ). The purpose of this study was to analyze effects of increased occlusal loads in adult animals. It was hypothesized that increased functional loading would cause degeneration of the mandibular condyle. Twelve female retired-breeder rabbits (mean age = three years, one month) were divided equally into three experimental groups and one control group. Each of the experimental rabbits received maxillary and mandibular light-cured anterior splints at the beginning of the study to create a bilateral posterior openbite (~2 mm). Theoretically, this should have resulted in increased loads to the TMJ. The first two groups wore the appliances for six and 12 weeks, respectively, prior to euthanasia. The third group wore the appliances for 12 weeks and then had them removed for 12 weeks prior to being euthanized. Fluorescent bone labels were utilized, and the specimens were analyzed histomorphometrically. The cartilage thickness was not statistically different between groups, nor were there any signs of cartilage degeneration. There was a trend, however, toward thinner condylar cartilage in the 12-week group. The six-week group showed significantly less labeled subchondral bone than the control group (0.5 ± 0.1 % versus 2.6 ± 0.3%, respectively; p<.05). The six- and 12-week groups both revealed significantly less periosteal surface label of the condylar neck than the control group (1.5 ± 0.8% and 5.3 ± 1. 7% versus 22.0 ± 3.5%, respectively; p<.05). Likewise, they both showed significantly more label at the cartilage-bone interface (1.0 ± 0.2% and 1.4 ± 0.2% versus 0.4 ± 0.2%, respectively; p<.05) and moderately more label along the trabecular surfaces than the control group. These results indicated that the increased loading of the mandibular condyle initially (at six weeks) depressed remodeling of the subchondral bone and decreased periosteal bone formation. Modeling increased at the cartilage-bone interface and potentially along the trabecular bone surfaces. With continued elevated occlusal loads (at 12 weeks), modeling still was quite active at the cartilage-bone interface and on the trabecular surfaces. Periosteal surface modeling remained depressed compared to the control group, but remodeling in the subchondral bone region increased to that of the control group. The 12/12-week group tended to show findings similar to the control group. This suggests that once loading was returned to normal, the condyle also returned toward preexperimental physiologic conditions. Although this study did not show the obvious degenerative changes one typically equates with osteoarthrosis, the elevated modeling at the cartilage-bone interface following increased joint loads was consistent with the early degenerative changes observed by Radin et al. in their joint-loading model. However, the rapid osseous changes seen in this study refute the theory that the adult TMJ is unable to adapt to altered functional loading.Item Antibody activity of rabbit macroglobulin(1967) Lindqvist, Kaare JulianItem Antigen binding by subunits of rabbit IgM antibody(1968) Coligan, John E.Item Bone Remodeling and Strain Variation Following Altered Mandibular Condyle Loading in Retired Breeder Rabbits(1993) Puntillo, Anthony M.; Garetto, Lawrence P.; Roberts, W. Eugene; Arbuckle, Gordon R.; Chen, Jie; Burr, David B.Several investigators have demonstrated modeling of the mandibular condyle foil following a change in load. A recent study evaluated the effect of age on the ability of the condyle to adapt to such a change. The present study explored the early changes in the mandibular condyles of retired breeder rabbits following an alteration in load, and attempted to quantify this load. Twelve female retired breeder New Zealand white rabbits were divided into four equal groups. Under general anesthesia strain gauges were placed on the lateral inferior body of the mandible bilaterally in two of the groups. Two days post-surgery acrylic splints were placed on the anterior teeth (resulting in a posterior open bite) of one the strain gauge groups and one group that did not receive strain gauges. The splints were maintained for 26 days. A control group received neither strain gauges nor splints. Intravital bone labels were administered to all groups to allow for histomorphometric analysis of condylar modeling and remodeling. In addition, principal strain measurements were recorded pre- and post-splint placement. The histomorphometric findings revealed a significant (p<.03) decrease in the subcondylar space of animals that received splints. Splinted animals also showed a significant increase in labeled surface area (p<.02) and in volume percent label (p<.05) of the trabeculae in the condylar neck region. In addition, surgical placement of the strain gauges significantly (p<.05) decreased the labeling of the periosteal surface in the neck region. The strain gauges proved functional in most rabbits for only a few days and registered large variations and no discernible differences in average maximum microstrain, and average change in microstrain. It was concluded from these results that an incisal prematurity (causing a posterior openbite), 26 days in duration, caused an increase functional load on the condyle. This load resulted in an increase in trabecular label and decrease in porosity of the subchondral plate. The decreased subcondylar space is possibly an indication of stiffening in this region. A stiffening of this nature has been suggested in previous studies to be a precursor to osteoarthritic degeneration.Item The carrier specificity of the anamnestic anti-sulfanilazo plaque forming cell response(1974) McCarthy, Robert C.Item Cell Kinetics of Osteoblast Histogenesis in Evolving Rabbit Secondary Haversian Systems Using a Double Labeling Technique with [³H]-Thymidine and Bromodeoxyuridine(1995) Sim, Yeongsuk; Roberts, Eugene; Garetto, Lawrence P.; Katona, Thomas R.; McDonald, James L.; Seifert, Mark F.The mechanism for internal cortical bone remodeling is orchestrated by the evolving secondary Haversian systems (SHSs), which originate on the surfaces of Volkmann's canals. During this coupled process, a cortical tunnel advances by the cutting cone of osteoclasts and closes by the bone-forming trail of osteoblasts. This study investigated the hypothesis that osteoblast histogenesis, within evolving SHSs of larger animals, is a vascular-related process, i.e., less differentiated osteogenic cells reside in close proximity to the advancing central blood vessel (CBV) while differentiating osteoblast precursors migrate toward the bone surface and become osteoblasts. Using a double-labeling method with [3 H]-thymidine and bromodeoxyuridine (BrdU), this study examined cell kinetics in 120 SHSs per rabbit at 12 hour intervals up to 72 hours after labeling. A total of 7 rabbits were injected with alizarin complexone (-1 O days: 20 mg/kg/day), tetracycline (-3 days: 10 mg/kg/day), [3H]-thymidine (time zero: 0.25 μCi/gm), and BrdU (1 hour before sacrifice: 25 mg/kg). The femoral midshaft was used for undecalcified fluorescent microscopic analysis of new bone vs. old bone and two adjacent diaphyseal tissues were demineralized for nuclear volume morphometric analysis of cells via light microscopy. Evolving SHSs demonstrating intense remodeling activity were selected for detailed cell kinetic analysis. The results showed that BrdU labeled cells were consistently located at the leading edge of the CBV (within 160 μm of its tip) and that the [3H]thymidine labeled cells were progressively left behind the advancing CBV (160 μm from the tip of CBV by 72 hours). The labeling indices (sampled 1 hour after labeling) between BrdU (10.6 ± 0.3 %) and [3H]-thymidine (14.4 ± 1.3 %) were comparable. Lightly labeled A+A' cells (identified as osteoprogenitor cells) remained in close approximation to the surface of the CBV (within 25 μm) and C+D cells (preosteoblasts) were located closer to bone-forming surfaces (~50 μm away from the CBV). The number of osteoblasts were increased up to 60 hours and about 22.5 ± 6.6 % of them survived to become osteocytes. The B cell compartment, characteristic of osteogenic tissues with a dense connective tissue component such as the periodontal ligament (POL), was essentially absent in the SHSs in this study. Although the direction of evolving SHSs was highly variable (caudally directed: 53.4 ± 11.2 % and rostrally directed: 41.6 ± 8.1 %), the osteogenic process along the advancing CBV was remarkably consistent. These results support the hypothesis that osteoblast histogenesis, associated with cortical bone remodeling, is a vascular-oriented differentiation process closely related to the internal angiogenesis within the evolving SHS. The primary proliferating region supporting osteogenesis was consistently located at the advancing tip of the CBV, suggesting the presence of a self-renewing, perivascular proliferative pool of cells accompanying the advancing vessel. In addition, a secondary proliferating region of cells trails the advancing CBV, providing for lateral migration of preosteoblasts to bone surfaces where they complete their development into functional osteoblasts. This study provides further insight into the similarities and differences in osteoblast histogenesis within evolving SHSs from adult rabbits and the more extensively studied rat POL model.Item Cyclosporine effects on rabbit lymphoid organs(1984) Jesseph, Jerry MichaelItem Effects of diabetes on adrenocortical function in the pregnant rabbit(1979) Guleff, Patricia S.Item Effects of indomethacin on lymphocyte populations in rabbit lymphoid tissues and peripheral blood(1991) Ennis, Keith Edward