Browsing by Subject "Plasmids"
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Item Fabrication and use of silicon hollow-needle arrays to achieve tissue nanotransfection in mouse tissue in vivo(Springer Nature, 2021) Xuan, Yi; Ghatak, Subhadip; Clark, Andrew; Li, Zhigang; Khanna, Savita; Pak, Dongmin; Agarwal, Mangilal; Roy, Sashwati; Duda, Peter; Sen, Chandan K.; Surgery, School of MedicineTissue nanotransfection (TNT) is an electromotive gene transfer technology that was developed to achieve tissue reprogramming in vivo. This protocol describes how to fabricate the required hardware, commonly referred to as a TNT chip, and use it for in vivo TNT. Silicon hollow-needle arrays for TNT applications are fabricated in a standardized and reproducible way. In <1 s, these silicon hollow-needle arrays can be used to deliver plasmids to a predetermined specific depth in murine skin in response to pulsed nanoporation. Tissue nanotransfection eliminates the need to use viral vectors, minimizing the risk of genomic integration or cell transformation. The TNT chip fabrication process typically takes 5-6 d, and in vivo TNT takes 30 min. This protocol does not require specific expertise beyond a clean room equipped for basic nanofabrication processes.Item Plasmid DNA in Neisseria gonorrohoeae(1975) Walker, Thomas StuartItem Plasmid DNA in the family Rhizobiaceae(1974) Klein, Gretchen E.Item A prokaryotic-eukaryotic shuttle vector(1983) Hoskins, Jo Ann