Browsing by Subject "Model Organisms"

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    CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice
    (Elsevier, 2023) Cassidy, Annelise; Pelletier, Stephane; Medical and Molecular Genetics, School of Medicine
    In this protocol, we describe the generation of conditional alleles in mice using the DECAI (DEgradation based on Cre-regulated Artificial Intron) approach. We detail steps for the CRISPR-mediated insertion of the short DECAI cassette within exon 3 of Scyl1 and the functional validation of alleles at genomic, transcriptomic, and protein levels. This strategy simplifies the process of generating mice with conditional alleles.
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    Generation of Casp8FL122/123GG Mice Using CRISPR-Cas9 Technology
    (Cell Press, 2020-12-18) Pelletier, Stephane; Tummers, Bart; Green, Douglas R.; Medical and Molecular Genetics, School of Medicine
    The purpose of this protocol is to describe the generation of missense mutations in mice using CRISPR-Cas9 technology. The current protocol focuses on the generation of a Casp8 FL122/123GG missense mutation, but it can be adapted to introduce any missense or nonsense mutation. For complete details on the use and execution of this protocol, please refer to Tummers et al. (2020).