Browsing by Subject "Magnetic"

Now showing 1 - 4 of 4
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    Analog Magnetic Levitation System
    (2024-05-03) Carlson, Evan; Martinez, Ricardo; Alkhalifah, Ali; Weissbach, Robert
    The Magnetic Levitation System operates as a closed-loop system, incorporating a photoresistor to provide feedback. The feedback mechanism enables the system to accurately ascertain the vertical position of the ball bearing within space.
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    Comparison of non-magnetic and magnetic beads multiplex assay for assessment of Plasmodium falciparum antibodies
    (PeerJ, 2019-01-03) Ondigo, Bartholomew N.; Park, Gregory S.; Ayieko, Cyrus; Nyangahu, Donald D.; Wasswa, Ronald; John, Chandy C.; Biochemistry and Molecular Biology, School of Medicine
    Background: New reagents have emerged allowing researchers to assess a growing number of vaccine-associated immune parameters. Multiplex immunoassay(s) are emerging as efficient high-throughput assays in malaria serology. Currently, commercial vendors market several bead reagents for cytometric bead assays (CBA) but relative performances are not well published. We have compared two types of bead-based multiplex assays to measure relative antibody levels to malarial antigens. Methods: Assays for the measurement of antibodies to five Plasmodium falciparum vaccine candidates using non-magnetic and magnetic fluorescent microspheres were compared for their performances with a Bio-Plex200 instrument. Mean fluorescence intensity (MFI) was determined from individuals from western Kenya and compared to known positive and negative control plasma samples. Results: P. falciparum recombinant antigens were successfully coupled to both non-magnetic and magnetic beads in multiplex assays. MFIs between the two bead types were comparable for all antigens tested. Bead recovery was superior with magnetic beads for all antigens. MFI values of stored non-magnetic coupled beads did not differ from freshly coupled beads, though they showed higher levels of bead aggregation. Discussion: Magnetic and non-magnetic beads performed similarly in P. falciparum antibody assays. Magnetic beads were more expensive, but had higher bead recovery, were more convenient to use, and provided rapid and easy protocol manipulation. Magnetic beads are a suitable alternative to non-magnetic beads in malarial antibody serology.
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    Magnetic Levitation System: Senior Project Report
    (2021-05-05) Martin, Cody A.; Snyder, Zachary S.; Weissbach, Robert S.
    This magnetic levitation system is designed to levitate a steel ball using an electromagnet suspended above it. The project was sponsored by Dr. Robert Weissbach, Chair of the Department of Engineering Technology at the Purdue School of Engineering Technology at IUPUI. This project was undertaken by undergraduate students Cody Martin and Zachary Snyder. This magnetic levitation system uses closed loop control with a digital controller to accomplish levitation.
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    Modeling of external self-excitation and force generation on magnetic nanoparticles inside vitreous cavity
    (AIMS Press, 2021) Parker, Evan; Mitchell, Chandler S.; Smith, Joshua P.; Carr, Evan; Akbari, Rasul; Izadian, Afshin; Hajrasouliha, Amir R.; Ophthalmology, School of Medicine
    The purpose of this manuscript was to design a better method for recovery from rhegmatogenous retinal detachment (RRD) surgery. We attempted to achieve this by designing a helmet that can manipulate intraocular magnetic nanoparticles (MNPs) and create a magnetic tamponade, eliminating the need for postoperative head positioning. A simulated analysis was developed to predict the pattern of magnetic force applied to the magnetic nanoparticles by external magnetic field. No participants were involved in this study. Instead, magnetic flux and force data for three different helmet designs were collected using virtual simulation tools. A prototype helmet was then constructed and magnetic flux and force data were recorded and compared to virtual data. For both virtual and physical scenarios, magnitude and direction of the resulting forces were compared to determine which design created the controlled direction and strongest forces into the back of the eye. Of the three virtual designs, both designs containing a visor had greater force magnitude than magnet alone. Between both designs with visors, the visor with bends resulted in forces more directed at the back of the eye. The physical prototype helmet shared similar measurements to virtual simulation with minimal percent error (Average = 5.47%, Standard deviation = 0.03). Of the three designs, the visor with bends generated stronger forces directed at the back of the eye, which is most appropriate for creating a tamponade on the retina. We believe that this design has shown promising capability for manipulating intraocular MNPs for the purpose of creating a tamponade for RRD.