Browsing by Subject "Lymphoid Progenitor Cells"

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    Implications of DPP4 modification of proteins that regulate stem/progenitor and more mature cell types
    (American Society of Hematology, 2013-07-11) Ou, Xuan; O'Leary, Heather A.; Broxmeyer, Hal E.; Microbiology & Immunology, IU School of Medicine
    Dipeptidylpeptidase (DPP) 4 has the potential to truncate proteins with a penultimate alanine, proline, or other selective amino acids at the N-terminus. DPP4 truncation of certain chemokines, colony-stimulating factors, and interleukins have recently been linked to regulation of hematopoietic stem/progenitor cells, more mature blood cells, and other cell types. We believe that the potential role of DPP4 in modification of many regulatory proteins, and their subsequent effects on numerous stem/progenitor and other cell-type functions has not been adequately appreciated. This review addresses the potential implications of the modifying effects of DPP4 on a large number of cytokines and other growth-regulating factors with either proven or putative DPP4 truncation sites on hematopoietic cells, and subsequent effects of DPP4-truncated proteins on multiple aspects of steady-state and stressed hematopoiesis, including stem/progenitor cell, and more mature cell, function.
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    Multipotent Hematopoietic Progenitors Divide Asymmetrically to Create Progenitors of the Lymphomyeloid and Erythromyeloid Lineages
    (Elsevier, 2014-10-23) Görgens, André; Ludwig, Anna-Kristin; Möllmann, Michael; Krawczyk, Adalbert; Dürig, Jan; Hanenberg, Helmut; Horn, Peter A.; Giebel, Bernd; Department of Pediatrics, IU School of Medicine
    Hematopoietic stem and progenitor cells (HSPCs) can self-renew and create committed progenitors, a process supposed to involve asymmetric cell divisions (ACDs). Previously, we had linked the kinetics of CD133 expression with ACDs but failed to detect asymmetric segregation of classical CD133 epitopes on fixed, mitotic HSPCs. Now, by using a novel anti-CD133 antibody (HC7), we confirmed the occurrence of asymmetric CD133 segregation on paraformaldehyde-fixed and living HSPCs. After showing that HC7 binding does not recognizably affect biological features of human HSPCs, we studied ACDs in different HSPC subtypes and determined the developmental potential of arising daughter cells at the single-cell level. Approximately 70% of the HSPCs of the multipotent progenitor (MPP) fraction studied performed ACDs, and about 25% generated lymphoid-primed multipotent progenitor (LMPP) as wells as erythromyeloid progenitor (EMP) daughter cells. Since MPPs hardly created daughter cells maintaining MPP characteristics, our data suggest that under conventional culture conditions, ACDs are lineage instructive rather than self-renewing., • The HC7 anti-CD133 antibody allows analyses of ACDs on fixed human HSPCs • HC7 and AC133 anti-CD133 antibodies allow tracking of CD133 in living HSPCs • Cells of the MPP fraction divide asymmetrically to create LMPP- and EMP-like cells • ACDs of MPPs are lineage instructive rather than self-renewing , In this article, Giebel, Görgens, and colleagues demonstrate at the single-cell level that most human hematopoietic progenitor cells with multipotent potential (MPPs) perform non-self-renewing cell divisions in vitro. With their first in vitro cell division, MPPs lose their multipotency and create pairs of differently specified daughter cells, one a lymphomyeloid-committed CD133+ progenitor and the other an erythromyeloid-committed CD133− progenitor.