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Item Amplified fragment length polymorphism analysis supports the valid separate species status of Lucilia caesar and L. illustris (Diptera: Calliphoridae)(Taylor & Francis:, 2017-12-08) Picard, Christine J.; Wells, Jeffrey D.; Ullyot, Anne; Rognes, Knut; Biology, School of ScienceCommon DNA-based species determination methods fail to distinguish some blow flies in the forensically and medically important genus Lucilia Robineau-Desvoidy. This is a practical problem, and it has also been interpreted as casting doubt on the validity of some morphologically defined species. An example is Lucilia illustris and L. caesar, which co-occur in Europe whilst only L. illustris has been collected in North America. Reports that these species shared both mitochondrial and nuclear gene sequences, along with claims that diagnostic morphological characters are difficult to interpret, were used to question their separate species status. We report here that amplified fragment length polymorphism profiles strongly support the validity of both species based on both assignment and phylogenetic analysis, and that traditional identification criteria based on male and female genital morphology are more reliable than has been claimed.Item Preparation, characterization, and application of a lipophilic coated exfoliated Egyptian blue for near-infrared luminescent latent fingermark detection(Forensic Chemistry, 2020-05-01) Shahbazi, Sorour; Goodpaster, John V.; Smith, Gregory D.; Becker, Thomas; Lewis, Simon W.A simple technique is described to create nanoscale near-infrared luminescent Egyptian blue pigment particles with lipophilic surfaces for latent fingermark detection. An exfoliated Egyptian blue powder coated with cetrimonium bromide was prepared using a simple, one-pot process. Particle size and surface coatings were characterized using scanning electron and atomic force microscopies, dynamic light scattering, and infrared and luminescence spectroscopies. This new fingermark dusting powder presents more contrast compared to uncoated Egyptian blue of comparable particle size as well as a commercial visible light fluorescent (Blitz Red) powder in the detection of aged fingermarks on model surfaces. The coated Egyptian blue pigment is a cost-effective fingermark dusting powder that reveals high contrast latent fingermarks with simple, inexpensive photography equipment.Item Studies into Exfoliation and Coating of Egyptian Blue for Application to the Detection of Latent Fingermarks(Chem Rxiv, 2020-09-23) Shahbazi, Sorour; Goodpaster, John V.; Smith, Gregory; Becker, Thomas; Lewis, Simon W.We have recently demonstrated that exfoliated Egyptian blue powder coated with cetrimonium bromide is effective for detecting latent fingermarks on a range of highly-patterned non-porous surfaces (see reference). In this extension of that work, we here present our preliminary studies into alternative approaches to preparing exfoliated Egyptian blue with a variety of different coatings. The various powders were then applied to the detection of latent fingermarks on non-porous surfaces. This proof of concept study demonstrated that there is potential improvement in performance compared to our previous work, however more comprehensive studies are required to compare the quality of the fingermarks developed with these powders against particles exfoliated in water.Item Studies into exfoliation and coating of Egyptian blue in methanol for application to the detection of latent fingermarks(Elsevier, 2022-07) Shahbazi, Sorour; Goodpaster, John V.; Smith, Gregory D.; Becker, Thomas; Lewis, Simon W.; Chemistry and Chemical Biology, School of ScienceWe have recently demonstrated that coated exfoliated Egyptian blue powder is effective for detecting latent fingermarks on a range of highly-patterned non-porous and semi-porous surfaces. In this extension of that work, we present our studies into an alternative approach to prepare exfoliated Egyptian blue coated with cetrimonium bromide and Tween® 20 using a simpler technique. The quality of the latent fingermarks developed with these exfoliated powders and the commercial powder were compared in a comprehensive study. Depletion series of natural fingermarks from a wide range of donors (12 males and females) deposited on non-porous (glass slides) and semi-porous (Australian banknotes) surfaces were used in this study. Enhancement in the performance of the coated exfoliated particles compared to the commercial powder was observed, particularly in the case of aged fingermarks and polymer banknotes as challenging substrates.