Browsing by Subject "Enzyme isocitrate dehydrogenase 1 (IDH1)"

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    Targeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibody
    (SpringerNature, 2017-04-03) Rizk, Shahir S.; Mukherjee, Somnath; Koide, Akiko; Koide, Shohei; Kossiakoff, Anthony A.; Department of Biochemistry and Molecular Biology, School of Medicine
    We have utilized a high-diversity phage display library to engineer antibody fragments (Fabs) that can modulate the activity of the enzyme isocitrate dehydrogenase 1 (IDH1). We show that a conformation-specific Fab can reactivate an IDH1 mutant associated with brain tumors. The results show that this strategy is a first step towards developing "activator drugs" for a large number of genetic disorders where mutations have disrupted protein function.