Browsing by Subject "Criticism"

Now showing 1 - 2 of 2
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    Borderland Journeys: A Layered Autoethnography
    (2014-02-25) Bankert-Countryman, Janice Elizabeth; Goering, Elizabeth M.; Dobris, Catherine A.; Rhodes, Nancy; Parrish-Sprowl, John
    The collection of pages spread before you now, this story-thesis, is a collection of stories about my journey from cult member to the place in life I am now, stories about those stories, and stories about the people who lived or read them, talked about them, and were changed by the tellings. Most importantly, the goal of this story-thesis is to illustrate how the process of story-making and -telling changes how we interpret our identities and our lifeworlds. I argue that the stories that we share change our identities, and I also argue that how we perceive our identity and the identities of others affects the stories that we share.
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    Comment on “Evidence that the ProPerDP method is inadequate for protein persulfidation detection due to lack of specificity”
    (American Association for the Advancement of Science, 2021-04-21) Dóka, Éva; Arnér, Elias S.J.; Schmidt, Edward E.; Dick, Tobias P.; van der Vliet, Albert; Yang, Jing; Szatmári, Réka; Ditrói, Tamás; Wallace, John L.; Cirino, Giuseppe; Olson, Kenneth; Motohashi, Hozumi; Fukuto, Jon M.; Pluth, Michael D.; Feelisch, Martin; Akaike, Takaaki; Wink, David A.; Ignarro, Louis J.; Nagy, Péter; Medicine, School of Medicine
    The recent report by Fan et al. alleged that the ProPerDP method is inadequate for the detection of protein persulfidation. Upon careful evaluation of their work, we conclude that the claim made by Fan et al. is not supported by their data, rather founded in methodological shortcomings. It is understood that the ProPerDP method generates a mixture of cysteine-containing and non–cysteine-containing peptides. Instead, Fan et al. suggested that the detection of non–cysteine-containing peptides indicates nonspecific alkylation at noncysteine residues. However, if true, then such peptides would not be released by reduction and therefore not appear as products in the reported workflow. Moreover, the authors’ biological assessment of ProPerDP using Escherichia coli mutants was based on assumptions that have not been confirmed by other methods. We conclude that Fan et al. did not rigorously assess the method and that ProPerDP remains a reliable approach for analyses of protein per/polysulfidation.