Browsing by Subject "Cell isolation"

Now showing 1 - 2 of 2
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    Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes
    (Elsevier, 2021-05-13) Guldner, Ian H.; Golomb, Samantha M.; Wang, Qingfei; Wang, Emilia; Zhang, Siyuan; Medicine, School of Medicine
    High dimensional compositional and transcriptional profiling of heterogeneous brain-infiltrating leukocytes can lead to novel biological and therapeutic discoveries. High-quality single-cell leukocyte preparations are a prerequisite for optimal single cell profiling. Here, we describe a protocol for epitope and RNA-preserving dissociation of adult mouse brains and subsequent leukocyte purification and staining, which is adaptable to homeostatic and pathogenic brains. Leukocyte preparation following this protocol permits exquisite single-cell surface protein and RNA profiling in applications including CyTOF and CITE-seq. For complete details on the use and execution of this protocol, please refer to Guldner et al. (2020) and Golomb et al. (2020).
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    Protocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood
    (Elsevier, 2024) Gutch, Sarah; Beasley, Lindsay; Cooper, Scott; Kaplan, Mark H.; Capitano, Maegan L.; Ropa, James; Microbiology and Immunology, School of Medicine
    Umbilical cord blood (CB) is a donor source for hematopoietic cell therapies. Understanding what drives hematopoietic stem and progenitor cell function is critical to our understanding of the usage of CB in hematopoietic cell therapies. Here, we describe how to isolate and analyze the function of human hematopoietic cells from umbilical CB. This protocol demonstrates assays that measure phenotypic properties and hematopoietic cell potency. For complete details on the use and execution of this protocol, please refer to Broxmeyer et al.