Browsing by Subject "Caries-activity"

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    A Potential Biofilm Metabolite Signature for Caries Activity - A Pilot Clinical Study
    (OMICS Publishing Group, 2015-03) Zandona, F.; Soini, H. A.; Novotny, M. V.; Santiago, E.; Eckert, G. J.; Preisser, J. S.; Benecha, H. K.; Arthur, R. A.; Zero, D. T.; Department of Cariology, Operative Dentistry and Dental Public Health, School of Dentistry
    BACKGROUND: This study's aim was to compare the dental biofilm metabolite-profile of caries-active (N=11) or caries-free (N=4) children by gas chromatography-mass spectrometry (GC/MS) analyses. METHODS: Samples collected after overnight fasting, with or without a previous glucose rinse, were combined for each child based on the caries status of the site, re-suspended in ethanol and analyzed by GC/MS. RESULTS: Biofilm from caries-active sites exhibited a different chromatographic profile compared to caries-free sites. Qualitative and quantitative analysis suggested a special cluster of branched alcohols and esters present at substantially higher intensity in biofilms of caries-active sites. CONCLUSIONS: This pilot study indicates that there are metabolites present in the biofilm which have the potential to provide a characteristic metabolomics signature for caries activity.