Browsing by Author "Yu, Rong"

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    Harnessing the NEON data revolution to advance open environmental science with a diverse and data-capable community
    (Wiley, 2021-12) Nagy, R. Chelsea; Balch, Jennifer K.; Bissell, Erin K.; Cattau, Megan E.; Glenn, Nancy F.; Halpern, Benjamin S.; Ilangakoon, Nayani; Johnson, Brian; Joseph, Maxwell B.; Marconi, Sergio; O’Riordan, Catherine; Sanovia, James; Swetnam, Tyson L.; Travis, William R.; Wasser, Leah A.; Woolner, Elizabeth; Zarnetske, Phoebe; Abdulrahim, Mujahid; Adler, John; Barnes, Grenville; Bartowitz, Kristina J.; Blake, Rachael E.; Bombaci, Sara P.; Brun, Julien; Buchanan, Jacob D.; Chadwick, K. Dana; Chapman, Melissa S.; Chong, Steven S.; Chung, Y. Anny; Corman, Jessica R.; Couret, Jannelle; Crispo, Erika; Doak, Thomas G.; Donnelly, Alison; Duffy, Katharyn A.; Dunning, Kelly H.; Duran, Sandra M.; Edmonds, Jennifer W.; Fairbanks, Dawson E.; Felton, Andrew J.; Florian, Christopher R.; Gann, Daniel; Gebhardt, Martha; Gill, Nathan S.; Gram, Wendy K.; Guo, Jessica S.; Harvey, Brian J.; Hayes, Katherine R.; Helmus, Matthew R.; Hensley, Robert T.; Hondula, Kelly L.; Huang, Tao; Hundertmark, Wiley J.; Iglesias, Virginia; Jacinthe, Pierre‐Andre; Jansen, Lara S.; Jarzyna, Marta A.; Johnson, Tiona M.; Jones, Katherine D.; Jones, Megan A.; Just, Michael G.; Kaddoura, Youssef O.; Kagawa‐Vivani, Aurora K.; Kaushik, Aleya; Keller, Adrienne B.; King, Katelyn B. S.; Kitzes, Justin; Koontz, Michael J.; Kouba, Paige V.; Kwan, Wai‐Yin; LaMontagne, Jalene M.; LaRue, Elizabeth A.; Li, Daijiang; Li, Bonan; Lin, Yang; Liptzin, Daniel; Long, William Alex; Mahood, Adam L.; Malloy, Samuel S.; Malone, Sparkle L.; McGlinchy, Joseph M.; Meier, Courtney L.; Melbourne, Brett A.; Mietkiewicz, Nathan; Morisette, Jeffery T.; Moustapha, Moussa; Muscarella, Chance; Musinsky, John; Muthukrishnan, Ranjan; Naithani, Kusum; Neely, Merrie; Norman, Kari; Parker, Stephanie M.; Perez Rocha, Mariana; Petri, Laís; Ramey, Colette A.; Record, Sydne; Rossi, Matthew W.; SanClements, Michael; Scholl, Victoria M.; Schweiger, Anna K.; Seyednasrollah, Bijan; Sihi, Debjani; Smith, Kathleen R.; Sokol, Eric R.; Spaulding, Sarah A.; Spiers, Anna I.; St. Denis, Lise A.; Staccone, Anika P.; Stack Whitney, Kaitlin; Stanitski, Diane M.; Stricker, Eva; Surasinghe, Thilina D.; Thomsen, Sarah K.; Vasek, Patrisse M.; Xiaolu, Li; Yang, Di; Yu, Rong; Yule, Kelsey M.; Zhu, Kai; Earth Sciences, School of Science
    It is a critical time to reflect on the National Ecological Observatory Network (NEON) science to date as well as envision what research can be done right now with NEON (and other) data and what training is needed to enable a diverse user community. NEON became fully operational in May 2019 and has pivoted from planning and construction to operation and maintenance. In this overview, the history of and foundational thinking around NEON are discussed. A framework of open science is described with a discussion of how NEON can be situated as part of a larger data constellation—across existing networks and different suites of ecological measurements and sensors. Next, a synthesis of early NEON science, based on >100 existing publications, funded proposal efforts, and emergent science at the very first NEON Science Summit (hosted by Earth Lab at the University of Colorado Boulder in October 2019) is provided. Key questions that the ecology community will address with NEON data in the next 10 yr are outlined, from understanding drivers of biodiversity across spatial and temporal scales to defining complex feedback mechanisms in human–environmental systems. Last, the essential elements needed to engage and support a diverse and inclusive NEON user community are highlighted: training resources and tools that are openly available, funding for broad community engagement initiatives, and a mechanism to share and advertise those opportunities. NEON users require both the skills to work with NEON data and the ecological or environmental science domain knowledge to understand and interpret them. This paper synthesizes early directions in the community’s use of NEON data, and opportunities for the next 10 yr of NEON operations in emergent science themes, open science best practices, education and training, and community building.
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    Novel Prostate-Specific Promoter Derived from PSA and PSMA Enhancers
    (Elsevier, 2002-09) Lee, Sang-Jin; Kim, Hong-Sup; Yu, Rong; Lee, KangRyul; Gardner, Thomas A.; Jung, Chaeyong; Jeng, Meei-Huey; Yeung, Fan; Cheng, Liang; Kao, Chinghai; Pathology and Laboratory Medicine, School of Medicine
    The expression of prostate-specific membrane antigen (PSMA) and prostate-specific antigen (PSA), two well characterized marker proteins, remains highly active in the hormone refractory stage of prostate cancer. In this study, an artificial chimeric enhancer (PSES) composed of two modified regulatory elements controlling the expression of PSA and PSMA genes was tested for its promoter activity and tissue specificity using the reporter system. As a result, this novel PSES promoter remained silent in PSA- and PSMA-negative prostate and non-prostate cancer cell lines, but mediated high levels of luciferase in PSA- and PSMA-expressing prostate cancer cell lines in the presence and absence of androgen. To determine whether PSES could be used for in vivo gene therapy of prostate cancer, a recombinant adenovirus, Ad-PSES-luc, was constructed. Luciferase activity in prostate cancer cell lines mediated by Ad-PSES-luc was 400- to 1000-fold higher than in several other non-prostate cell lines, suggesting the high tissue-specificity of the PSES promoter in an adenoviral vector. Finally, recombinant virus Ad-PSES-luc was injected into mice to evaluate the tissue-discriminatory promoter activity in an experimental animal. Unlike Ad-CMV-luc, the luciferase activity from systemic injection of Ad-PSES-luc was fairly low in all major organs. However, when injected into prostate, Ad-PSES-luc drove high luciferase activity almost exclusively in prostate and not in other tissues. Our results demonstrated the potential use of PSES for the treatment of androgen-independent prostate cancer patients.