Browsing by Author "Yoshida, Junko"

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    Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans
    (The American Society for Clinical Investigation, 2023-03-08) Lin, Yang; Banno, Kimihiko; Gil, Chang-Hyun; Myslinski, Jered; Hato, Takashi; Shelley, William C.; Gao, Hongyu; Xuei, Xiaoling; Basile, David P.; Yoshimoto, Momoko; Prasain, Nutan; Tarnawsky, Stefan P.; Adams, Ralf H.; Naruse, Katsuhiko; Yoshida, Junko; Murphy, Michael P.; Horie, Kyoji; Yoder, Mervin C.; Pediatrics, School of Medicine
    Most circulating endothelial cells are apoptotic, but rare circulating endothelial colony-forming cells (C-ECFCs), also known as blood outgrowth endothelial cells, with proliferative and vasculogenic activity can be cultured; however, the origin and naive function of these C-ECFCs remains obscure. Herein, detailed lineage tracing revealed murine C-ECFCs emerged in the early postnatal period, displayed high vasculogenic potential with enriched frequency of clonal proliferative cells compared with tissue-resident ECFCs, and were not committed to or derived from the BM hematopoietic system but from tissue-resident ECFCs. In humans, C-ECFCs were present in the CD34bright cord blood mononuclear subset, possessed proliferative potential and in vivo vasculogenic function in a naive or cultured state, and displayed a single cell transcriptome sharing some umbilical venous endothelial cell features, such as a higher protein C receptor and extracellular matrix gene expression. This study provides an advance for the field by identifying the origin, naive function, and antigens to prospectively isolate C-ECFCs for translational studies.