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Browsing by Author "Tang, Bo"
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Item Identification of miPEP133 as a novel tumor-suppressor microprotein encoded by miR-34a pri-miRNA(BMC, 2020-09-14) Kang, Min; Tang, Bo; Li, Jixi; Zhou, Ziyan; Liu, Kang; Wang, Rensheng; Jiang, Ziyan; Bi, Fangfang; Patrick, David; Kim, Dongin; Mitra, Anirban K.; Yang-Hartwich, Yang; Medical and Molecular Genetics, School of MedicineBackground Very few proteins encoded by the presumed non-coding RNA transcripts have been identified. Their cellular functions remain largely unknown. This study identifies the tumor-suppressor function of a novel microprotein encoded by the precursor of miR-34a. It consists of 133 amino acid residues, thereby named as miPEP133 (pri-microRNA encoded peptide 133). Methods We overexpressed miPEP133 in nasopharyngeal carcinoma (NPC), ovarian cancer and cervical cancer cell lines to determine its effects on cell growth, apoptosis, migration, or invasion. Its impact on tumor growth was evaluated in a xenograft NPC model. Its prognostic value was analyzed using NPC clinical samples. We also conducted western blot, immunoprecipitation, mass spectrometry, confocal microscopy and flow cytometry to determine the underlying mechanisms of miPEP133 function and regulation. Results miPEP133 was expressed in normal human colon, stomach, ovary, uterus and pharynx. It was downregulated in cancer cell lines and tumors. miPEP133 overexpression induced apoptosis in cancer cells and inhibited their migration and invasion. miPEP133 inhibited tumor growth in vivo. Low miPEP133 expression was an unfavorable prognostic marker associated with advanced metastatic NPC. Wild-type p53 but not mutant p53 induced miPEP133 expression. miPEP133 enhanced p53 transcriptional activation and miR-34a expression. miPEP133 localized in the mitochondria to interact with mitochondrial heat shock protein 70kD (HSPA9) and prevent HSPA9 from interacting with its binding partners, leading to the decrease of mitochondrial membrane potential and mitochondrial mass. Conclusion miPEP133 is a tumor suppressor localized in the mitochondria. It is a potential prognostic marker and therapeutic target for multiple types of cancers.Item Obtain a Simulation Model of a Pedestrian Collision Imminent Braking System Based on the Vehicle Testing Data(IEEE, 2014-10) Tang, Bo; Chien, Stanley; Chen, Yaobin; Electrical and Computer Engineering, School of Engineering and TechnologyForward pedestrian collision imminent braking (CIB) systems has proven to be of great significance in improving road safety and protecting pedestrians. Since pedestrian CIB technology is not mature, the performance of different pedestrian CIB systems varies significantly. Therefore the simulation of a CIB system needs to be vehicle specific. The CIB simulation can be based on the component sensor parameters and decision making rules. Since these parameters and decision rules for on the market vehicles are not available outside of vehicle manufactures, it is difficult for the general research communities to develop a good CIB simulation model based on this approach. To solve this problem, this study presents a new method for developing a pedestrian CIB simulation model using pedestrian CIB testing data. The implementation was in PreScan. The simulation results demonstrate that a pedestrian CIB simulation model developed using this methodology could reflect the behavior of a real vehicle equipped with pedestrian CIB system.Item Pedestrian Protection Using the Integration of V2V and the Pedestrian Automatic Emergency Braking System(IEEE, 2016-11) Tang, Bo; Chien, Stanley; Huang, Zhi; Chen, Yaobin; Department of Electrical and Computer Engineering, School of Engineering and TechnologyVehicle to Vehicle (V2V) communication systems enable vehicles to communicate with each other and use the shared information to make safety related decisions. However, the safety improvement of the current V2V systems only benefits V2V-enabled objects in the V2V network. The Pedestrian Automatic Emergency Braking System (PAEB) can utilize onboard sensors to detect pedestrians and make safety related actions so it benefits the individual vehicle and the pedestrians detected by its PAEB. To further improve pedestrian safety, the idea for integrating the capabilities of V2V and PAEB (V2V-PAEB) has been proposed, which allows the information of pedestrians detected by onboard sensors of a vehicle to be shared in the V2V network. A V2V-PAEB enabled vehicle uses not only its onboard sensors, but also received V2V messages from others to detect potential collisions with pedestrians and make better safety related decisions. In this paper, a Matlab/Simulink based simulation model of V2V-PAEB system is presented for demonstrating the proper architecture and information processing processes, and for providing the quick start of developing a better simulation model of V2V-PAEB. The proposed model has also been tested in PreScan simulation environment.Item Pedestrian Protection Using the Integration of V2V Communication and Pedestrian Automatic Emergency Braking System(2015-12-01) Tang, Bo; Chien, Stanley Yung-Ping; Chen, Yaobin; Li, Lingxi; King, BrianThe Pedestrian Automatic Emergency Braking System (PAEB) can utilize on-board sensors to detect pedestrians and take safety related actions. However, PAEB system only benefits the individual vehicle and the pedestrians detected by its PAEB. Additionally, due to the range limitations of PAEB sensors and speed limitations of sensory data processing, PAEB system often cannot detect or do not have sufficient time to respond to a potential crash with pedestrians. For further improving pedestrian safety, we proposed the idea for integrating the complimentary capabilities of V2V and PAEB (V2V-PAEB), which allows the vehicles to share the information of pedestrians detected by PAEB system in the V2V network. So a V2V-PAEB enabled vehicle uses not only its on-board sensors of the PAEB system, but also the received V2V messages from other vehicles to detect potential collisions with pedestrians and make better safety related decisions. In this thesis, we discussed the architecture and the information processing stages of the V2V-PAEB system. In addition, a comprehensive Matlab/Simulink based simulation model of the V2V-PAEB system is also developed in PreScan simulation environment. The simulation result shows that this simulation model works properly and the V2V-PAEB system can improve pedestrian safety significantly.