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Browsing by Author "Takahashi, Kazuhisa"
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Item Comparison of the clinical performance and usefulness of five SARS-CoV-2 antibody tests(PloS, 2021) Wakita, Mitsuru; Idei, Mayumi; Saito, Kaori; Horiuchi, Yuki; Yamatani, Kotoko; Ishikawa, Suzuka; Yamamoto, Takamasa; Igawa, Gene; Hinata, Masanobu; Kadota, Katsuhiko; Kurosawa, Taro; Takahashi, Sho; Saito, Takumi; Misawa, Shigeki; Akazawa, Chihiro; Naito, Toshio; Milda, Takashi; Takahashi, Kazuhisa; Ai, Tomohiko; Tabe, Yoko; Medicine, School of MedicineWe examined the usefulness of five COVID-19 antibody detection tests using 114 serum samples at various time points from 34 Japanese COVID-19 patients. We examined Elecsys Anti-SARS-CoV-2 from Roche, and four immunochromatography tests from Hangzhou Laihe Biotech, Artron Laboratories, Chil, and Nadal. In the first week after onset, Elecsys had 40% positivity in Group S (severe cases) but was negative in Group M (mild-moderate cases). The immunochromatography kits showed 40–60% and 0–8% positivity in Groups S and M, respectively. In the second week, Elecsys showed 75% and 50% positivity, and the immunochromatography tests showed 5–80% and 50–75% positivity in Groups S and M, respectively. After the third week, Elecsys showed 100% positivity in both groups. The immunochromatography kits showed 100% positivity in Group S. In Group M, positivity decreased to 50% for Chil and 75–89% for Artron and Lyher. Elecsys and immunochromatography kits had 91–100% specificity. Elecsys had comparable chronological change of cut-off index values in the two groups from the second week to the sixth week. The current SARS-CoV-2 antibody detection tests do not provide meaningful interpretation of severity and infection status. Its use might be limited to short-term epidemiological studies.Item Periostin-related progression of different types of experimental pulmonary hypertension: A role for M2 macrophage and FGF-2 signalling(Wiley, 2022) Yoshida, Takashi; Nagaoka, Tetsutaro; Nagata, Yuichi; Suzuki, Yoshifumi; Tsutsumi, Takeo; Kuriyama, Sachiko; Watanabe, Junko; Togo, Shinsaku; Takahashi, Fumiyuki; Matsushita, Masakazu; Joki, Yusuke; Konishi, Hakuoh; Nunomura, Satoshi; Izuhara, Kenji; Conway, Simon J.; Takahashi, Kazuhisa; Pediatrics, School of MedicineBackground and objective: Remodelling of pulmonary arteries (PA) contributes to the progression of pulmonary hypertension (PH). Periostin, a matricellular protein, has been reported to be involved in the development of PH. We examined the role of periostin in the pathogenesis of PH using different types of experimental PH. Methods: PH was induced by vascular endothelial growth factor receptor antagonist (Sugen5416) plus hypoxic exposure (SuHx) and venous injection of monocrotaline-pyrrole (MCT-P) in wild-type (WT) and periostin-/- mice. Pulmonary haemodynamics, PA remodelling, expression of chemokines and fibroblast growth factor (FGF)-2, accumulation of macrophages to small PA and the right ventricle (RV) were examined in PH-induced WT and periostin-/- mice. Additionally, the role of periostin in the migration of macrophages, human PA smooth muscle (HPASMCs) and endothelial cells (HPMVECs) was investigated. Results: In PH induced by SuHx and MCT-P, PH and accumulation of M2 macrophage to small PA were attenuated in periostin-/- mice. PA remodelling post-SuHx treatment was also mild in periostin-/- mice compared to WT mice. Expression of macrophage-associated chemokines and FGF-2 in lung tissue, and accumulation of CD68-positive cells in the RV were less in SuHx periostin-/- than in SuHx WT mice. Periostin secretion in HPASMCs and HPMVECs was enhanced by transforming growth factor-β. Periostin also augmented macrophage, HPASMCs and HPMVECs migration. Separately, serum periostin levels were significantly elevated in patients with PH compared to healthy controls. Conclusion: Periostin is involved in the development of different types of experimental PH, and may also contribute to the pathogenesis of human PH.