Browsing by Author "Southern, Kathleen"

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    A guide to selecting high-performing antibodies for PLC-gamma-2 for use in Western Blot, immunoprecipitation and immunofluorescence
    (Taylor & Francis, 2024-01-18) Ruíz Moleón, Vera; Fotouhi, Maryam; Alende, Charles; Ayoubi, Riham; Bedford, Logan M.; Southern, Kathleen; Richardson, Timothy I.; Laflamme, Carl; NeuroSGC/YCharOS/EDDU collaborative group; ABIF consortium; Pharmacology and Toxicology, School of Medicine
    Phosphatidylinositol-specific phospholipase C gamma 2 (PLC-gamma-2) is an enzyme that regulates the function of immune cells. PLC-gamma-2 has been implicated in neurodegenerative and autoimmune disorders, yet investigation of this protein has been limited by a lack of independently characterized antibodies. Here we have characterized eleven PLC-gamma-2 commercial antibodies for use in Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.