Browsing by Author "Olopade, Olufunmilayo I."

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    Breast Cancer Germline Genetic Counseling and Testing for Populations of African Heritage Globally: A Scoping Review on Research, Practice, and Bioethical Considerations
    (American Society of Clinical Oncology, 2023) Iwai, Yoshiko; Toumbo, Kadiata; Zuze, Takondwa; Morgan, Jenny S.; Simwinga, Lusayo; Wright, Sarah T.; Fedoriw, Yuri; Oladeru, Oluwadamilola T.; Balogun, Onyinye D.; Roberson, Mya L.; Olopade, Olufunmilayo I.; Tomoka, Tamiwe; Elmore, Shekinah N. C.; Community and Global Health, Richard M. Fairbanks School of Public Health
    Purpose: Despite the disproportionately high risk of breast cancer among women of African heritage, little is known about the facilitators and barriers to implementing germline genetic testing and counseling (GT/C). Methods: This scoping review followed guidelines recommended by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for scoping reviews. Published manuscripts from database inception through 2021 were sourced from PubMed, Cumulative Index to Nursing and Allied Health Literature via EBSCO, Embase, Cochrane Library, and Scopus. Search terms were used to retrieve articles addressing (1) African heritage, (2) breast cancer, and (3) GT or GC. The screening involved abstract and title review and full-text review. Data were extracted for all articles meeting the inclusion criteria. Results: A total of 154 studies were included. Most studies that took place were conducted in the United States (71.4%), and most first authors (76.9%) were from the United States. GT was conducted in 73 (49.7%) studies. BRCA1/BRCA2 were the most commonly studied genes for germline mutations. GC was conducted in 49 studies (33.3%), and perspectives on GC were evaluated in 43 (29.3%). The use of racial/ethnic categories varied broadly, although African American was most common (40.1%). Racism was mentioned in three studies (2.0%). Conclusion: There is a growing body of literature on GT/C for breast cancer in women of African heritage. Future studies on GT/C of African populations should consider increased clarity around racial/ethnic categorizations, continued community engagement, and intentional processes for informed consent.
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    Effects of Slide Storage on Detection of Molecular Markers by IHC and FISH in Endometrial Cancer Tissues From a Clinical Trial: An NRG Oncology/GOG Pilot Study
    (Wolters Kluwer, 2022) Grushko, Tatyana A.; Filiaci, Virginia L.; Montag, Anthony G.; Apushkin, Marsha; Gomez, Maria J.; Monovich, Laura; Ramirez, Nilsa C.; Schwab, Carlton; Kesterson, Joshua P.; Seward, Shelly M.; Method, Michael W.; Olopade, Olufunmilayo I.; Fleming, Gini F.; Birrer, Michael J.; Medicine, School of Medicine
    We performed a pilot study in anticipation of using long-aged precut formalin fixed paraffin embedded (FFPE) tissue sections stored in real-world conditions for translational biomarker studies of TOP2A, Ki67, and HER2 in endometrial cancer. FFPE tissue blocks or unstained slides or both from GOG-0177 were collected centrally (1999-2000) and stored at room temperature. During 2004-2011 specimens were stored at 4°C. Matched pairs of stored slides and freshly cut slides from stored blocks were analyzed for TOP2A (KiS1), Ki67 (MIB1) and HER2 (Herceptest™) proteins. To assess DNA stability (HER2 PathVision), FISH was repeated on stored slides from 21 cases previously shown to be HER2-amplified. IHC staining intensity and extent, mean FISH copies/cell, and copy number ratios were compared using the kappa statistic for concordance or signed rank test for differences in old cut versus new cut slides. IHC results reflected some protein degradation in stored slides. The proportion of cells with TOP2A staining was lower on average by 12% in older sections (p=.03). The proportion of Ki67 positive cells was lower in stored slides by an average of 10% (p<.01). Too few cases in the IHC cohort were FISH positive for any conclusions. HER2 amplification by FISH was unaffected by slide storage. We conclude that use of aged stored slides for proliferation markers TOP2A and Ki67 is feasible but may modestly underestimate true values in endometrial cancer. Pilot studies for particular storage conditions/durations/antigens to be used in translational studies are warranted.
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    Proceedings of the 8th Annual Conference on the Science of Dissemination and Implementation
    (BioMed Central, 2016-08-01) Chambers, David; Simpson, Lisa; Hill-Briggs, Felicia; Neta, Gila; Vinson, Cynthia; Beidas, Rinad; Marcus, Steven; Aarons, Gregory; Hoagwood, Kimberly; Schoenwald, Sonja; Evans, Arthur; Hurford, Matthew; Rubin, Ronnie; Hadley, Trevor; Barg, Frances; Walsh, Lucia; Adams, Danielle; Mandell, David; Martin, Lindsey; Mignogna, Joseph; Mott, Juliette; Hundt, Natalie; Kauth, Michael; Kunik, Mark; Naik, Aanand; Cully, Jeffrey; McGuire, Alan; White, Dominique; Bartholomew, Tom; McGrew, John H.; Luther, Lauren; Rollins, Angie; Salyers, Michelle P.; Cooper, Brittany; Funaiole, Angie; Richards, Julie; Lee, Amy; Lapham, Gwen; Caldeiro, Ryan; Lozano, Paula; Gildred, Tory; Achtmeyer, Carol; Ludman, Evette; Addis, Megan; Marx, Larry; Bradley, Katharine; VanDeinse, Tonya; Wilson, Amy Blank; Stacey, Burgin; Powell, Byron; Bunger, Alicia; Cuddeback, Gary; Barnett, Miya; Stadnick, Nicole; Brookman-Frazee, Lauren; Lau, Anna; Dorsey, Shannon; Pullmann, Michael; Mitchell, Shannon; Schwartz, Robert; Kirk, Arethusa; Dusek, Kristi; Oros, Marla; Hosler, Colleen; Gryczynski, Jan; Barbosa, Carolina; Dunlap, Laura; Lounsbury, David; O'Grady, Kevin; Brown, Barry; Damschroder, Laura; Waltz, Thomas; Powell, Byron; Ritchie, Mona; Waltz, Thomas; Atkins, David; Imel, Zac E.; Xiao, Bo; Can, Doğan; Georiou, Panayiotis; Narayanan, Shrikanth; Berkel, Cady; Gallo, Carlos; Sandler, Irwin; Brown, C. Hendricks; Wolcik, Sharlene; Mauricio, Anne Marie; Gallo, Carlos; Mehrota, Sanjay; Chandurkar, Dharmendra; Bora, Siddhartha; Das, Arup; Tripathi, Anand; Saggurti, Nirajan; Raj, Anita; Hughes, Eric; Jacobs, Brian; Kirkendall, Eric; Loeb, Danielle; Trinkley, Katy; Yang, Michael; Sprowell, Andrew; Nease, Donald; Lyon, Aaron; Lewis, Cara; Boyd, Meredith; Melvin, Abigail; Nicodimos, Semret; Liu, Freda; Jungbluth, Nathanial; Lyon, Aaron; Landis-Lewis, Zach; Sales, Anne; Baloh, Jure; Ward, Marcia; Zhu, Xi; Bennett, Ian; Unutzer, Jurgen; Mao, Johnny; Proctor, Enola; Vredevoogd, Mindy; Chan, Ya-Fen; Williams, Nathaniel; Green, Phillip; Bernstein, Steven; Rosner, June-Marie; DeWitt, Michelle; Tetrault, Jeanette; Dziura, James; Hsiao, Allen; Sussman, Scott; O'Connor, Patrick; Toll, Benjamin; Jones, Michael; Gassaway, Julie; Tobin, Jonathan; Zatzick, Douglas; Bradbury, Angela R.; Patrick-Miller, Linda; Egleston, Brian; Olopade, Olufunmilayo I.; Hall, Michael J.; Daly, Mary B.; Fleisher, Linda; Grana, Generosa; Ganschow, Pamela; Fetzer, Dominique; Brandt, Amanda; Farengo-Clark, Dana; Forman, Andrea; Gaber, Rikki S.; Gulden, Cassandra; Horte, Janice; Long, Jessica; Chambers, Rachelle Lorenz; Lucas, Terra; Madaan, Shreshtha; Mattie, Kristin; McKenna, Danielle; Montgomery, Susan; Nielsen, Sarah; Powers, Jacquelyn; Rainey, Kim; Rybak, Christina; Savage, Michelle; Seelaus, Christina; Stoll, Jessica; Stopfer, Jill; Yao, Shirley; Domchek, Susan; Hahn, Erin; Munoz-Plaza, Corrine; Wang, Jianjin; Delgadillo, Jazmine Garcia; Mittman, Brian; Gould, Michael; Liang, Shuting (Lily); Kegler, Michelle C.; Cotter, Megan; Philips, Emily; Hermstad, April; Morton, Rentonia; Beasley, Derrick; Martinez, Jeremy; Riehman, Kara; Gustafson, David; Marsch, Lisa; Mares, Louise; Quanbeck, Andrew; McTavish, Fiona; McDowell, Helene; Brown, Randall; Thomas, Chantelle; Glass, Joseph; Isham, Joseph; Shah, Dhavan; Liebschutz, Jane; Lasser, Karen; Watkins, Katherine; Ober, Allison; Hunter, Sarah; Lamp, Karen; Ewing, Brett; Iwelunmor, Juliet; Gyamfi, Joyce; Blackstone, Sarah; Quakyi, Nana Kofi; Plange-Rhule, Jacob; Ogedegbe, Gbenga; Kumar, Pritika; Devanter, Nancy Van; Nguyen, Nam; Nguyen, Linh; Nguyen, Trang; Phuong, Nguyet; Shelley, Donna; Rudge, Sian; Langlois, Etienne; Tricco, Andrea; Ball, Sherry; Lambert-Kerzner, Anne; Sulc, Christine; Simmons, Carol; Shell-Boyd, Jeneen; Oestreich, Taryn; O'Connor, Ashley; Neely, Emily; McCreight, Marina; Labebue, Amy; DiFiore, Doreen; Brostow, Diana; Ho, P. Michael; Aron, David; Harvey, Jillian; McHugh, Megan; Scanon, Dennis; Lee, Rebecca; Soltero, Erica; Parker, Nathan; McNeill, Lorna; Ledoux, Tracey; McIsaac, Jessie-Lee; MacLeod, Kate; Ata, Nicole; Jarvis, Sherry; Kirk, Sara; Purtle, Jonathan; Dodson, Elizabeth; Brownson, Ross; Mittman, Brian; Curran, Geoffrey; Pyne, Jeffrey; Aarons, Gregory; Ehrhart, Mark; Torres, Elisa; Miech, Edward; Stevens, Kathleen; Hamilton, Alison; Cohen, Deborah; Padgett, Deborah; Morshed, Alexandra; Patel, Rupa; Prusaczyk, Beth; Aron, David C.; Gupta, Divya; Ball, Sherry; Hand, Rosa; Abram, Jenica; Wolfram, Taylor; Hastings, Molly; Moreland-Russell, Sarah; Tabek, Rachel; Ramsey, Alex; Baumann, Ana; Kryzer, Emily; Montgomery, Katherine; Lewis, Ericka; Padek, Margaret; Brownson, Ross; Mamaril, Cezar Brian; Mays, Glen; Branham, Keith; Timsina, Lava; Mays, Glen; Hogg, Rachel; Fagan, Abigail; Shapiro, Valerie; Brown, Eric; Haggerty, Kevin; Hawkins, David; Oesterle, Sabrina; Hawkins, David; Catalano, Richard; McKay, Virginia; Dolcini, M. Margaret; Hoffer, Lee; Moin, Tannaz; Li, Jinnan; Duru, O. Kenrik; Ettner, Susan; Turk, Norman; Chan, Charles; Keckhafer, Abigail; Luchs, Robert; Ho, Sam; Mangione, Carol; Selby, Peter; Zawertailo, Laurie; Minian, Nadia; Balliunas, Dolly; Dragonetti, Rosa; Hussain, Sarwar; Lecce, Julia; Chinman, Matthew; Acosta, Joie; Ebener, Patricia; Malone, Patrick S.; Slaughter, Mary; Freedman, Darcy; Flocke, Susan; Lee, Eunlye; Matlack, Kristen; Trapl, Erika; Ohri-Vachaspati, Punam; Taggart, Morgan; Borawski, Elaine; Parrish, Amanda; Harris, Jeffrey; Kohn, Marlana; Hammerback, Kristen; McMilan, Becca; Hannon, Peggy; Swindle, Taren; Curran, Geoffrey; Whiteside-Mansell, Leanne; Ward, Wendy; Holt, Cheryl; Santos, Sheri Lou; Tagai, Erin; Scheirer, Mary Ann; Carter, Roxanne; Bowie, Janice; Haider, Muhiuddin; Slade, Jimmie; Wang, Min Qi; Masica, Andrew; Ogola, Gerald; Berryman, Candice; Richter, Kathleen; Shelton, Rachel; Jandorf, Lina; Erwin, Deborah; Truong, Khoa; Javier, Joyce R.; Coffey, Dean; Schrager, Sheree; Palinkas, Lawrence; Miranda, Jeanne; Johnson, Veda; Hutcherson, Valerie; Ellis, Ruth; Kharmats, Anna; Marshall-King, Sandra; LaPradd, Monica; Fonseca-Becker, Fannie; Kepka, Deanna; Bodson, Julia; Warner, Echo; Fowler, Brynn; Shenkman, Elizabeth; Hogan, William; Odedina, Folakami; Leon, Jessica De; Hooper, Monica; Carrasquillo, Olveen; Reams, Renee; Hurt, Myra; Smit, Steven; Szapocznik, Jose; Nelson, David; Mandal, Prabir; Teufel, James; Department of Psychology, School of Science
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    β-Catenin is required for the tumorigenic behavior of triple-negative breast cancer cells
    (PLoS, 2015-02-06) Xu, Jinhua; Prosperi, Jenifer R.; Choudhury, Noura; Olopade, Olufunmilayo I.; Goss, Kathleen H.; Department of Biochemistry and Molecular Biology, IU School of Medicine
    Our previous data illustrated that activation of the canonical Wnt signaling pathway was enriched in triple-negative breast cancer and associated with reduced overall survival in all patients. To determine whether Wnt signaling may be a promising therapeutic target for triple-negative breast cancer, we investigated whether β-catenin was necessary for tumorigenic behaviors in vivo and in vitro. β-catenin expression level was significantly reduced in two human triple-negative breast cancer cell lines, MDA-MB-231 and HCC38, using lentiviral delivery of β-catenin-specific small hairpin RNAs (shRNAs). Upon implantation of the cells in the mammary fat pad of immunocompromised mice, we found that β-catenin shRNA HCC38 cells formed markedly smaller tumors than control cells and grew much more slowly. In in vitro assays, β-catenin silencing significantly reduced the percentage of Aldefluor-positive cells, a read-out of the stem-like cell population, as well as the expression of stem cell-related target genes including Bmi-1 and c-Myc. β-catenin-knockdown cells were also significantly impaired in their ability to migrate in wound-filling assays and form anchorage-independent colonies in soft agar. β-catenin-knockdown cells were more sensitive to chemotherapeutic agents doxorubicin and cisplatin. Collectively, these data suggest that β-catenin is required for triple-negative breast cancer development by controlling numerous tumor-associated properties, such as migration, stemness, anchorage-independent growth and chemosensitivity.