Browsing by Author "Miller, David"

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    Changes in mRNA/protein expression and signaling pathways in in vivo passaged mouse ovarian cancer cells
    (Public Library of Science, 2018-06-21) Cai, Qingchun; Fan, Qipeng; Buechlein, Aaron; Miller, David; Nephew, Kenneth P.; Liu, Sheng; Wan, Jun; Xu, Yan; Obstetrics and Gynecology, School of Medicine
    The cure rate for late stage epithelial ovarian cancer (EOC) has not significantly improved over several decades. New and more effective targets and treatment modalities are urgently needed. RNA-seq analyses of a syngeneic EOC cell pair, representing more and less aggressive tumor cells in vivo were conducted. Bioinformatics analyses of the RNA-seq data and biological signaling and function studies have identified new targets, such as ZIP4 in EOC. Many up-regulated tumor promoting signaling pathways have been identified which are mainly grouped into three cellular activities: 1) cell proliferation and apoptosis resistance; 2) cell skeleton and adhesion changes; and 3) carbohydrate metabolic reprograming. Unexpectedly, lipid metabolism has been the major down-regulated signaling pathway in the more aggressive EOC cells. In addition, we found that hypoxic responsive genes were at the center stage of regulation and detected functional changes were related to cancer stem cell-like activities. Moreover, our genetic, cellular, biochemical, and lipidomic analyses indicated that cells grown in 2D vs. 3D, or attached vs. suspended had dramatic changes. The important clinical implications of peritoneal cavity floating tumor cells are supported by the data proved in this work. Overall, the RNA-seq data provide a landscape of gene expression alterations during tumor progression.
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    Federal Policing Structures - Mexico and Comparisons, ID: DipLab1927216
    (2019-12-17) Anderson, Cortney; Ichita, Maurice; Palmer, Alexandria; Kuglarz, Jordyn; Clemens, Zach; Miller, David; Powers, Terry; Zagone, Danielle; Hamernik, Madison; Keller, John; Conway, Scott; Rosas, Selena; Tate, Angela; Foley, William A., Jr.
    This report examines Mexico’s drug and police culture while analyzing police cultures in similarly organized countries. Drugs and cartels have a long and violent history within Mexico and the problem is spilling over the border into the United States. With corrupt and insufficiently trained police forces, the ability to effectively fight the drug war is extremely limited. Both the United States and Mexico have worked together to try to combat these connected issues, but more needs to be done. Key recommendations were found by analyzing the bipartite structures in Brazil and South Africa with a focus on Armenia. These recommendations include the following: - Prioritize the interests of the people - Standardized training - Distinguish a disciplinary authority to investigate human rights violations - Practice community-based policing using well-trained officers - Senior National Guard positions not to be reserved for friends of political appointees - Improve communication and information sharing - U.S. Technology and systems recommendations - Appoint a cabinet level Drug Czar * Limitations to this research include the use of only online materials and database articles and journals.
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    A Threat Assessment and Security Analysis of the Three Sports Facilities of Indiana University-Purdue University, Indianapolis NCAA Softball Fields, Carroll Stadium, and the IU Natatorium
    (2019-05-06) Diaz, Carmen; Haines, Amanda; Richardson, Owen; Brooks, Willie; Ward, Kristol; Albert, Kevin; Amos, Tania; Baker, A. J.; Beck, Alex; Clemens, Zachary; Conway, Scott; Lane, Emelyne; Lopez, Sergio; McDonald, Douglas; Miller, David; Newcomer, John; Palmer, Alexandria; Plunkett, Abbie; Purkiser, Ryan; Smith, Andrew; Sutton, Troy; Foley, William A., Jr.
    This research report provides a security assessment of the Softball Fields, Carroll Stadium, and the Natatorium Complex at Indiana University-Purdue University Indianapolis (IUPUI). The purpose of this report is to prevent and mitigate harm to visitors and these facilities which resulting from human-made or natural disasters. Research is guided by the hypothesis that these facilities- due to their respective importance, locations, and attendance patterns are in harm’s way; and that certain strategies of prevention, protection, and mitigation coupled with effective preparedness, response, and recovery can lessen risk, improve security and provide A THREAT ASSESSMENT AND SECURITY ANALYSIS 5 added resilience. Further, “harm’s way” is considered to be either a natural disaster or a human-made disaster, accident, active provocation, or act of terrorism. Methods of analysis include applied research; predominantly utilizing qualitative data with some quantitative investigation. Results of this assessment illustrate that these venues possess numerous vulnerabilities to both natural and human-made threats that if exposed, could result in serious consequences. The two most likely natural hazards identified include straight-line winds and tornadoes. Further, the most likely human threats to these facilities arise from a potential terrorist vehicle attack (TVA) and an active shooter. This project also identifies a specific need for additional planning to prevent an IED or VBIED attack on the Natatorium. Common themes from the attached three case studies reveal that given theses vulnerabilities, the following safety and security adjustments are recommended:  Surveillance equipment  Metal detectors  Security bollards or other temporary barriers  Evacuation routes and shelter in place plans  Special event security procedures  Weather related technology and protocols  Staff training for emergency situations