Browsing by Author "Mervis, Carolyn B."

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    Syntaxin1A overexpression and pain insensitivity in individuals with 7q11.23 duplication syndrome
    (American Society for Clinical Investigation, 2024-02-22) Iadarola, Michael J.; Sapio, Matthew R.; Loydpierson, Amelia J.; Mervis, Carolyn B.; Fehrenbacher, Jill C.; Vasko, Michael R.; Maric, Dragan; Eisenberg, Daniel P.; Nash, Tiffany A.; Kippenhan, J. Shane; Garvey, Madeline H.; Mannes, Andrew J.; Gregory, Michael D.; Berman, Karen F.; Pharmacology and Toxicology, School of Medicine
    Genetic modifications leading to pain insensitivity phenotypes, while rare, provide invaluable insights into the molecular biology of pain and reveal targets for analgesic drugs. Pain insensitivity typically results from Mendelian loss-of-function mutations in genes expressed in nociceptive (pain-sensing) dorsal root ganglion (DRG) neurons that connect the body to the spinal cord. We document a pain insensitivity mechanism arising from gene overexpression in individuals with the rare 7q11.23 duplication syndrome (Dup7), who have 3 copies of the approximately 1.5-megabase Williams syndrome (WS) critical region. Based on parental accounts and pain ratings, people with Dup7, mainly children in this study, are pain insensitive following serious injury to skin, bones, teeth, or viscera. In contrast, diploid siblings (2 copies of the WS critical region) and individuals with WS (1 copy) show standard reactions to painful events. A converging series of human assessments and cross-species cell biological and transcriptomic studies identified 1 likely candidate in the WS critical region, STX1A, as underlying the pain insensitivity phenotype. STX1A codes for the synaptic vesicle fusion protein syntaxin1A. Excess syntaxin1A was demonstrated to compromise neuropeptide exocytosis from nociceptive DRG neurons. Taken together, these data indicate a mechanism for producing “genetic analgesia” in Dup7 and offer previously untargeted routes to pain control.