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Browsing by Author "McCoy, Morgan H."
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Item Answer to August 2014 Photo Quiz(American Society for Microbiology, 2014-08) Relich, Ryan F.; Faught, Phillip R.; McCoy, Morgan H.; Alexander, Riley E.; Pritt, Bobbi S.; Pathology and Laboratory Medicine, School of MedicineItem Answer to August 2016 Photo Quiz(American Society for Microbiology, 2016-08) Relich, Ryan F.; Boyd, Kathleen M.; McCoy, Morgan H.; Kaufman, Cynthia; Simpson, Edward R.; Christenson, John C.; Department of Pathology and Laboratory Medicine, IU School of MedicineItem Photo Quiz: An Unexpected Colonoscopic Finding in a 13-Year-Old Boy(American Society for Microbiology (ASM), 2014-08) Relich, Ryan F.; Faught, Phillip R.; McCoy, Morgan H.; Alexander, Riley E.; Pritt, Bobbi S.; Department of Pathology & Laboratory Medicine, IU School of MedicineItem Photo Quiz: Fever, Rash, and Polyarthralgia in a 5-Year-Old Male(American Society for Microbiology, 2016-08) Relich, Ryan F.; Boyd, Kathleen M.; McCoy, Morgan H.; Kaufman, Cynthia; Simpson, Edward R.; Christenson, John C.; Department of Pathology and Laboratory Medicine, IU School of MedicineItem qPCR is a sensitive and rapid method for detection of cytomegaloviral DNA in formalin-fixed, paraffin-embedded biopsy tissue(MyJoVE Corporation, 2014-07-09) McCoy, Morgan H.; Post, Kristin; Sen, Joyashree D.; Chang, Hsim Y.; Zhao, Zijin; Fan, Rong; Chen, Shaoxiong; Leland, Diane; Cheng, Liang; Lin, Jingmei; Department of Pathology and Laboratory Medicine, IU School of MedicineIt is crucial to identify cytomegalovirus (CMV) infection in the gastrointestinal (GI) tract of immunosuppressed patients, given their greater risk for developing severe infection. Many laboratory methods for the detection of CMV infection have been developed, including serology, viral culture, and molecular methods. Often, these methods reflect systemic involvement with CMV and do not specifically identify local tissue involvement. Therefore, detection of CMV infection in the GI tract is frequently done by traditional histology of biopsy tissue. Hematoxylin and eosin (H&E) staining in conjunction with immunohistochemistry (IHC) have remained the mainstays of examining these biopsies. H&E and IHC sometimes result in atypical (equivocal) staining patterns, making interpretation difficult. It was shown that quantitative polymerase chain reaction (qPCR) for CMV can successfully be performed on formalin-fixed, paraffin-embedded (FFPE) biopsy tissue for very high sensitivity and specificity. The goal of this protocol is to demonstrate how to perform qPCR testing for the detection of CMV in FFPE biopsy tissue in a clinical laboratory setting. This method is likely to be of great benefit for patients in cases of equivocal staining for CMV in GI biopsies.