Browsing by Author "Liu, Zhiqiang"

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    In vitro and in vivo inhibition of the host TRPC4 channel attenuates Zika virus infection
    (EMBO Press, 2024) Chen, Xingjuan; Yan, Yunzheng; Liu, Zhiqiang; Yang, Shaokang; Li, Wei; Wang, Zhuang; Wang, Mengyuan; Guo, Juan; Li, Zhenyang; Zhu, Weiyan; Yang, Jingjing; Yin, Jiye; Dai, Qingsong; Li, Yuexiang; Wang, Cui; Zhao, Lei; Yang, Xiaotong; Guo, Xiaojia; Leng, Ling; Xu, Jiaxi; Obukhov, Alexander G.; Cao, Ruiyuan; Zhong, Wu; Anatomy, Cell Biology and Physiology, School of Medicine
    Zika virus (ZIKV) infection may lead to severe neurological consequences, including seizures, and early infancy death. However, the involved mechanisms are still largely unknown. TRPC channels play an important role in regulating nervous system excitability and are implicated in seizure development. We investigated whether TRPCs might be involved in the pathogenesis of ZIKV infection. We found that ZIKV infection increases TRPC4 expression in host cells via the interaction between the ZIKV-NS3 protein and CaMKII, enhancing TRPC4-mediated calcium influx. Pharmacological inhibition of CaMKII decreased both pCREB and TRPC4 protein levels, whereas the suppression of either TRPC4 or CaMKII improved the survival rate of ZIKV-infected cells and reduced viral protein production, likely by impeding the replication phase of the viral life cycle. TRPC4 or CaMKII inhibitors also reduced seizures and increased the survival of ZIKV-infected neonatal mice and blocked the spread of ZIKV in brain organoids derived from human-induced pluripotent stem cells. These findings suggest that targeting CaMKII or TRPC4 may offer a promising approach for developing novel anti-ZIKV therapies, capable of preventing ZIKV-associated seizures and death.
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    Periodontal health: A national cross‐sectional study of knowledge, attitudes and practices for the public oral health strategy in China
    (Wiley, 2019-04) Zhao, Qian; Wang, Shi-Bin; Xu, Guodong; Song, Yiqing; Han, Xiaozhe; Liu, Zhiqiang; Zhou, Xuan; Zhang, Tianyi; Huang, Kewu; Yang, Ting; Lin, Yingxiang; Wu, Sinan; Wang, Zuomin; Wang, Chen; Epidemiology, School of Public Health
    Aim To assess the status of periodontal health knowledge, attitudes and practices (KAP) among Chinese adults. Materials and Methods A cross‐sectional study was conducted in a nationally representative sample of adults (N = 50,991) aged 20 years or older from ten provinces, autonomous regions, and municipalities. Percentages of Chinese adults with correct periodontal knowledge, positive periodontal attitudes, and practices were estimated. Multiple logistic regression analyses were used to examine the related factors. Results Less than 20% of Chinese adults were knowledgeable about periodontal disease. Very few (2.6%) of Chinese adults use dental floss ≥once a day and undergo scaling ≥once a year and visit a dentist (6.4%) in the case of gingival bleeding. Periodontal health KAP was associated with gender, age, body mass index, marital status, place of residence, education level, income, smoking status, and history of periodontal disease. Conclusions Periodontal health KAP are generally poor among the Chinese adult population. Community‐based health strategies to improve periodontal health KAP need to be implemented. Increasing knowledge of periodontal disease, the cultivation of correct practices in response to gingival bleeding, and the development of good habits concerning the use of dental floss and regular scaling should be public oral health priorities.
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    Systemic Oxidative Stress Biomarkers in Chronic Periodontitis: A Meta-Analysis
    (Hindawi Publishing Corporation, 2014-11-16) Liu, Zhiqiang; Liu, Yan; Song, Yiqing; Zhang, Xi; Wang, Songlin; Wang, Zuomin; Department of Epidemiology, Richard M. Fairbanks School of Public Health
    Oxidative stress biomarkers have been observed in peripheral blood of chronic periodontitis patients; however, their associations with periodontitis were not consistent. This meta-analysis was performed to clarify the associations between chronic periodontitis and oxidative biomarkers in systemic circulation. Electronic searches of PubMed and Embase databases were performed until October 2014 and articles were selected to meet inclusion criteria. Data of oxidative biomarkers levels in peripheral blood of periodontitis patients and periodontal healthy controls were extracted to calculate standardized mean differences (SMDs) and 95% confidence intervals (CIs) by using random-effects model. Of 31 eligible articles, 16 articles with available data were included in meta-analysis. Our results showed that periodontitis patients had significantly lower levels of total antioxidant capacity (SMD = −2.02; 95% CI: −3.08, −0.96; P = 0.000) and higher levels of malondialdehyde (SMD = 0.99; 95% CI: 0.12, 1.86; P = 0.026) and nitric oxide (SMD = 4.98; 95% CI: 2.33, 7.63; P = 0.000) than periodontal healthy control. Superoxide dismutase levels between two groups were not significantly different (SMD = −1.72; 95% CI: −3.50, 0.07; P = 0.059). In conclusion, our meta-analysis showed that chronic periodontitis is significantly associated with circulating levels of three oxidative stress biomarkers, indicating a role of chronic periodontitis in systemic diseases.