Browsing by Author "Li, Libo"

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    Correction to: Probing the multimodal fungiform papilla: complex peripheral nerve endings of chorda tympani taste and mechanosensitive fibers before and after Hedgehog pathway inhibition
    (Springer, 2022) Donnelly, Christopher R.; Kumari, Archana; Li, Libo; Vesela, Iva; Bradley, Robert M.; Mistretta, Charlotte M.; Pierchala, Brian A.; Anatomy, Cell Biology and Physiology, School of Medicine
    This corrects the article "Probing the multimodal fungiform papilla: complex peripheral nerve endings of chorda tympani taste and mechanosensitive fibers before and after Hedgehog pathway inhibition" in Cell Tissue Res, volume 387 on page 225.
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    Education Matters: Longitudinal Pathways to Midlife Heavy Drinking in a National Cohort of Black Americans
    (Wiley, 2022) Mulia, Nina; Witbrodt, Jane; Karriker-Jaffe, Katherine J.; Li, Libo; Lui, Camillia K.; Zapolski, Tamika; Psychology, School of Science
    Aims To estimate longitudinal pathways from childhood socioeconomic position (SEP) to educational attainment and midlife heavy drinking in Black Americans in order to identify potential points of early intervention to reduce risk for alcohol-related problems in adulthood. Design, Setting, Participants Data are from 1,299 Black Americans in the US National Longitudinal Survey of Youth, followed from 1979 (ages 15-19) through 2012. Given gender differences in factors related to education and alcohol outcomes, gender-stratified path models were analyzed. Measurements Youth socioeconomic indicators included parental education (approximating childhood SEP) and adolescent poverty duration. Education-related measures included high-poverty school, perceived school safety, academic problems, suspension from school, educational expectations, and educational attainment. Adulthood measures included repeated unemployment, poverty duration, and mean frequency of heavy drinking (6+ drinks/day) in young adulthood and midlife. Covariates included age, dual-parent household, marital status, early drinking onset, and family history of alcohol problems. Findings For both genders, two main pathways originating from low childhood SEP flowed to educational attainment through (1) educational expectations and (2) suspension, and from educational attainment to midlife heavy drinking (total indirect effect = 0.131 (95% confidence interval [CI]: .072-.197) for women, and 0.080 (.035-.139) for men). For both genders, adolescent poverty (standardized βs >.135), academic problems (βs >.220), and school suspension (βs >.165) were significantly (ps <.05) related to lower educational expectations. In adulthood, educational attainment was indirectly protective against midlife heavy drinking through its significant effects (ps <.05) on young adult heavy drinking for both genders (βs < -.200) and economic hardships for women (βs < -.290). Conclusions Low childhood socioeconomic position among Black Americans appears to be associated with subsequent, adverse socioeconomic and school experiences that lead to lower educational attainment and, ultimately, greater heavy drinking at midlife. Interventions that mitigate these earlier, adverse experiences might have indirect effects on midlife heavy drinking.
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    Probing the multimodal fungiform papilla: complex peripheral nerve endings of chorda tympani taste and mechanosensitive fibers before and after Hedgehog pathway inhibition
    (Springer, 2022) Donnelly, Christopher R.; Kumari, Archana; Li, Libo; Vesela, Iva; Bradley, Robert M.; Mistretta, Charlotte M.; Pierchala, Brian A.; Anatomy, Cell Biology and Physiology, School of Medicine
    The fungiform papilla (FP) is a gustatory and somatosensory structure incorporating chorda tympani (CT) nerve fibers that innervate taste buds (TB) and also contain somatosensory endings for touch and temperature. Hedgehog (HH) pathway inhibition eliminates TB, but CT innervation remains in the FP. Importantly, after HH inhibition, CT neurophysiological responses to taste stimuli are eliminated, but tactile responses remain. To examine CT fibers that respond to tactile stimuli in the absence of TB, we used Phox2b-Cre; Rosa26LSL-TdTomato reporter mice to selectively label CT fibers with TdTomato. Normally CT fibers project in a compact bundle directly into TB, but after HH pathway inhibition, CT fibers reorganize and expand just under the FP epithelium where TB were. This widened expanse of CT fibers coexpresses Synapsin-1, β-tubulin, S100, and neurofilaments. Further, GAP43 expression in these fibers suggests they are actively remodeling. Interestingly, CT fibers have complex terminals within the apical FP epithelium and in perigemmal locations in the FP apex. These extragemmal fibers remain after HH pathway inhibition. To identify tactile end organs in FP, we used a K20 antibody to label Merkel cells. In control mice, K20 was expressed in TB cells and at the base of epithelial ridges outside of FP. After HH pathway inhibition, K20 + cells remained in epithelial ridges but were eliminated in the apical FP without TB. These data suggest that the complex, extragemmal nerve endings within and disbursed under the apical FP are the mechanosensitive nerve endings of the CT that remain after HH pathway inhibition.