Browsing by Author "Kyazike, Sharifah"

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    Activation of Natural Killer Cell by Lunasin and Cytokine
    (Office of the Vice Chancellor for Research, 2014-04-11) Kyazike, Sharifah; Lewis, David; Tung, Chun-Yu; Han, Ling; Chang, Hua-Chen
    Cancer immunotherapy is one of the emerging therapeutic strategies to harness the immune system to eradicate chemotherapy-resistant cancerous cells. NK cells can recognize and eliminate cancer cells before adaptive immunity is developed. Human NK cells can be divided into 2 major subsets based on their surface expression of CD56. NK cells with CD56 bright populations are major cytokine producers, while NK cells expressing CD56 dim have higher lytic activity. Due to the role of NK cells in cancer surveillance, any approach to enhance their activity may augment cancer treatment. We have recently shown that soypeptide Lunasin is a novel immune modulating agent that, together with cytokines, enhances IFN- γ and Granzyme B expression by NK cells. This synergism augments the natural cytotoxicity of NK cells against various tumors in vitro as well as in the xenograft model. The objective of this study is to evaluate the effects of Lunasin on antibody-dependent cellular cytotoxicity (ADCC) activity of NK cells against Rituximab-coated human B-lymphoma Raji cells. We also evaluated the expression of several markers involved in NK-mediated tumorcidal activity using flow cytometry. Together, these results suggest that Lunasin could enhance the efficacy of NK cell-based immunotherapy for cancer.
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    Comparison of Proteins and Genes Sequences among Different Species to Highlight Similarity
    (Office of the Vice Chancellor for Research, 2013-04-05) Kyazike, Sharifah
    Some amphibians have the unique abilities of regeneration which is lost in humans and other mammalians. Investigations from previous research show that the mechanism of the amphibian ability to regenerate is not fully understood. The central purpose of our investigation is to address our hypothesis which says that high level of EV15 is essential to delay entry of axolotl limb cells into M phase until they are fully dedifferentiated, and that the loss of regeneration capacity in developing Xenopus limbs is due to loss of the capacity to enforce this delay. As part of this project, my major goal is to compare two or more protein sequences with the purpose of highlighting hormonology region among the different species sequences. This study is helpful because it helps compare unknown species sequence such as axolotl against other known proteins to determine high hormonology region. The proteins I will align are YAP, Yorkie, P53, MST1/2, LAT1/2, and SAV1. The tool I plan to use for the sequence comparison is ClustalW2 software. The results from the sequence comparison will be used to assign primers or antibody of genes and proteins, which will then be used to investigate the unique abilities of amphibian regeneration.