Browsing by Author "Jiang, X."

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    LacSwitch® II Regulation of Connexin43 cDNA Expression Enables Gap-Junction Single-Channel Analysis
    (Future Science, 2003-05) Zhong, G.; Mantel, P.L.; Jiang, X.; Jarry-Guichard, T.; Gros, D.; Labarrere, C.; Moreno, A.P.; Medicine, School of Medicine
    Metabolic and electrical coupling through gap junction channels is implicated in cell differentiation, tissue homeostasis, and electrotonic propagation of signals in excitable tissues. The characterization of gating properties of these channels requires electrophysiological recordings at both single- and multiple-channel levels. Hence, a system that is able to control connexin expression by external means would provide a useful tool. To regulate the expression of connexins in cells, plasmids encoding a transactivator and/or a lac-operon IPTG response-dependent Cx43 target gene were transfected into communication-deficient N2a neuroblastoma cells. Immunoblotting, dye coupling, and electrophysiological methods revealed that expression of Cx43 in selected clones could be tightly regulated. After 15–20 h of acute induction with IPTG, cell-to-cell communication reached its peak with junctional conductances of 15–30 nS. Chronic induction at specific doses of IPTG produced constant, controlled levels of Cx43 expression, which were reflected by predictable junctional coupling levels. These conditions allowed prolonged recordings from either lowly or highly coupled cells, making lac operon an ideal regulatory system for channel gating studies at a single-channel level.
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    Nonvolatile voltage controlled molecular spin state switching
    (AIP, 2019) Hao, G.; Mosey, A.; Jiang, X.; Yost, A. J.; Sapkota, K. R.; Wang, G. T.; Zhang, X.; Zhang, J.; N’Diaye, A. T.; Cheng, R.; Xu, X.; Dowben, P. A.; Physics, School of Science
    Voltage-controlled room temperature isothermal reversible spin crossover switching of [Fe{H2B(pz)2}2(bipy)] thin films is demonstrated. This isothermal switching is evident in thin film bilayer structures where the molecular spin crossover film is adjacent to a molecular ferroelectric. The adjacent molecular ferroelectric, either polyvinylidene fluoride hexafluoropropylene or croconic acid (C5H2O5), appears to lock the spin crossover [Fe{H2B(pz)2}2(bipy)] molecular complex largely in the low or high spin state depending on the direction of ferroelectric polarization. In both a planar two terminal diode structure and a transistor structure, the voltage controlled isothermal reversible spin crossover switching of [Fe{H2B(pz)2}2(bipy)] is accompanied by a resistance change and is seen to be nonvolatile, i.e., retained in the absence of an applied electric field. The result appears general, as the voltage controlled nonvolatile switching can be made to work with two different molecular ferroelectrics: croconic acid and polyvinylidene fluoride hexafluoropropylene.