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Browsing by Author "Jiang, Gening"
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Item Single-cell transcriptome and antigen-immunoglobin analysis reveals the diversity of B cells in non-small cell lung cancer(BMC, 2020-06-24) Chen, Jian; Tan, Yun; Sun, Fenghuan; Hou, Likun; Zhang, Chi; Ge, Tao; Yu, Huansha; Wu, Chunxiao; Zhu, Yuming; Duan, Liang; Wu, Liang; Song, Nan; Zhang, Liping; Zhang, Wei; Wang, Di; Chen, Chang; Wu, Chunyan; Jiang, Gening; Zhang, Peng; Medical and Molecular Genetics, School of MedicineBackground Malignant transformation and progression of cancer are driven by the co-evolution of cancer cells and their dysregulated tumor microenvironment (TME). Recent studies on immunotherapy demonstrate the efficacy in reverting the anti-tumoral function of T cells, highlighting the therapeutic potential in targeting certain cell types in TME. However, the functions of other immune cell types remain largely unexplored. Results We conduct a single-cell RNA-seq analysis of cells isolated from tumor tissue samples of non-small cell lung cancer (NSCLC) patients, and identify subtypes of tumor-infiltrated B cells and their diverse functions in the progression of NSCLC. Flow cytometry and immunohistochemistry experiments on two independent cohorts confirm the co-existence of the two major subtypes of B cells, namely the naïve-like and plasma-like B cells. The naïve-like B cells are decreased in advanced NSCLC, and their lower level is associated with poor prognosis. Co-culture of isolated naïve-like B cells from NSCLC patients with two lung cancer cell lines demonstrate that the naïve-like B cells suppress the growth of lung cancer cells by secreting four factors negatively regulating the cell growth. We also demonstrate that the plasma-like B cells inhibit cancer cell growth in the early stage of NSCLC, but promote cell growth in the advanced stage of NSCLC. The roles of the plasma-like B cell produced immunoglobulins, and their interacting proteins in the progression of NSCLC are further validated by proteomics data. Conclusion Our analysis reveals versatile functions of tumor-infiltrating B cells and their potential clinical implications in NSCLC.Item Society for Translational Medicine Expert consensus on the selection of surgical approaches in the management of thoracic esophageal carcinoma(AME Publishing Company, 2019-01) Mao, Yousheng; Yu, Zhentao; You, Bin; Fang, Wentao; Badgwell, Brian; Berry, Mark F.; Ceppa, DuyKhanh P.; Chen, Chun; Chen, Haiquan; Cuesta, Miguel A.; D’Journo, Xavier Benoit; Eslick, Guy D.; Fu, Jianhua; Fu, Xiangning; Gao, Shugeng; He, Jianxing; He, Jie; Huang, Yunchao; Jiang, Gening; Jiang, Zhongmin; Kim, Jae Y.; Li, Danqing; Li, Hui; Li, Shanqing; Liu, Deruo; Liu, Lunxu; Liu, Yongyu; Li, Xiaofei; Li, Yin; Mao, Weimin; Molena, Daniela; Morse, Christopher R.; Novoa, Nuria M.; Tan, Lijie; Tan, Qunyou; Toker, Alper; Tong, Ti; Wang, Qun; Weksler, Benny; Xu, Lin; Xu, Shidong; Yan, Tiansheng; Zhang, Lanjun; Zhang, Xingyi; Zhang, Xun; Zhang, Zhu; Zhi, Xiuyi; Zhou, Qinghua; Department of Surgery, Indiana University School of Medicine