Browsing by Author "Ivaturi, Samantha"

Now showing 1 - 3 of 3
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    Development of Cancer-Related Protease Assay Using DNA-Encoded Proteomic Probes
    (2015-05) Ivaturi, Samantha; Jetson, Rachael; Krusemark, Casey
    Proteases function by breaking the peptide bonds within proteins that hold amino acids together. This action, proteolysis, is essential for the proper functioning of numerous biological processes. The activity of this vast group of enzymes is held in a delicate balance in normal living systems, however tumor growth and metastasis can develop as a result of irregular protease activity. A number of protease activities are well known to be misrelated in cancerous cells. The objective of the project is to develop DNA-encoded probes to use as a tool for protease assays that will enable low-cost, high-throughput profiling of enzymatic activity in samples by DNA sequence analysis for the early detection, characterization, and prevention of cancer. The encoding of functional proteomic information in DNA sequence promises to have significant benefits over traditional methods, including highly sensitive detection and capabilities for extensive multiplexing. Model proteases used in this experiment will include caspase 3 and prostate specific antigen (PSA).
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    Protocol for RNA Primer Building: Hawkins Lab 2021
    (2021-07) Ivaturi, Samantha; Sowers, Adrianne; Willhite, Sydney
    Step-by-step protocol for building customized and optimized primers for select RNA sequences using NCBI and Primer3Web available software.
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    Quality of Life after Palliative Pelvic Exenteration for Gynecologic Malignancy
    (2021-02) Villiger, Nicole; Ivaturi, Samantha; Mishra, Abhipri; Mueller, Melissa
    Case Summary: Patient is a 44yo female with metastatic vulvar squamous cell carcinoma (SCC) diagnosed after 1-year history of a vulvar lesion, initially treated with radiation and radical vulvectomy. Following vulvectomy she developed an enlarging chronic wound, exposing her pubic ramus. Biopsies revealed recurrent SCC and osteomyelitis of the pubic ramus. Pain associated with the wound severely limited her ability to sit and ambulate. A tumor board decided to proceed with a palliative total pelvic exenteration 21 months after initial diagnosis of SCC for pain relief, including removal of the uterus, right fallopian tube and ovary, bladder, distal ureters, vagina, rectum, pubic symphysis, and pubic rami. Perineal reconstruction was completed with left pedicled anterolateral thigh flap with pedicled vastus lateralis flap. Unfortunately, she developed severe left hip pain due to persistent osteomyelitis and was diagnosed with locally recurrent SCC a few months after surgery.