Browsing by Author "Ho-A-Lim, Kimberly T."

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    Differentiation and Three-dimensional Organization of Retinal Ganglion Cells using Human Induced Pluripotent Stem Cells
    (Office of the Vice Chancellor for Research, 2015-04-17) Ho-A-Lim, Kimberly T.; Ohlemacher, Sarah K.; Meyer, Jason S.
    Retinal Ganglion Cells (RGCs) are a type of neuron which function to relay visual messages between the retina and brain, and are characterized by their long axons which form part of the optic nerve. Dysfunction in this communication pathway is highly implicated in degenerative blinding disorders such as glaucoma. Unique applications using human induced pluripotent stem cells (hiPSCs) offer the ability to model human diseases, and potentially develop novel therapeutic approaches to rescue or replace damaged cells. In order to better understand the progression of degenerative eye diseases, a remaining challenge is to precisely identify the sequence of events which contribute to the diseased state, and how their features differ from non-diseased cells. Efforts were therefore undertaken to visually document the maturation of RGCs by analyzing their morphology and three-dimension organization at varying stages of development. Induced retinal cells were harvested at six different stages of development and fixed in 4% paraformaldehyde (PFA) solution to arrest their development. Cells were then cryoprotected in combinations of sucrose and Optimal Cutting Temperature (OCT) solutions, and frozen using powered dry ice. Following cryostat sectioning, samples were subject to immunocytochemistry staining to visualize for retinal-like organization of cells. Preliminary results have indicated the presence of the RGC marker Brn3, as well as markers for other retinal cell types. Future tests intend to characterize these retinal cell types according to their morphology and three-dimensional organization.
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    HEALTH DISPARITIES: THE GENETIC CONTRIBUTION IN THE AFRICAN AMERICAN COMMUNITY
    (Office of the Vice Chancellor for Research, 2012-04-13) Ho-A-Lim, Kimberly T.; Henry-Anthony, Ronda C.
    Since the completion of the Human Genome Project, it has been found that genes and their function play a role in 9 out of 10 of the leading causes of death in the U.S. Some of these causes such as heart disease, cancer, stroke and diabetes are significantly prevalent in the African American community. African Americans often experience the largest differences in health risks when compared to their White counterparts. This research project will examine how mutated genes and their function, contribute to health disparities in the African American community. The population for this research project will only include individuals of African ancestry born in the U.S. A brief survey will be conducted to inquire about participants’ knowledge of genetics and its influence on disease inheritance. The data collected will be interpreted as a representation of average African Americans’ knowledge of genetic influences on disease inheritance. Additionally, data will be obtained from facilities that offer genetic testing services. Specifically, I hope to obtain information on the racial populations who utilize these services, primarily, those with higher occurrences of genetic disorders. Coupled with the survey’s data, I will use the testing centers’ information to determine whether a correlation exists between the following variables: knowledge of genetics, use of genetic testing services and prevalence of inheritable diseases. I expect a strong correlation between afore mentioned variables. My hypothesis is that this correlation will prove undetected gene mutations when inherited, contribute to health disparities in the African American community.