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Browsing by Author "Hansen, Jens"
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Item Gene editing reverses arrhythmia susceptibility in humanized PLN-R14del mice: modelling a European cardiomyopathy with global impact(Oxford University Press, 2022) Dave, Jaydev; Raad, Nour; Mittal, Nishka; Zhang, Lu; Fargnoli, Anthony; Oh, Jae Gyun; Savoia, Maria Elisabetta; Hansen, Jens; Fava, Marika; Yin, Xiaoke; Theofilatos, Konstantinos; Ceholski, Delaine; Kohlbrenner, Erik; Jeong, Dongtak; Wills, Lauren; Nonnenmacher, Mathieu; Haghighi, Kobra; Costa, Kevin D.; Turnbull, Irene C.; Mayr, Manuel; Cai, Chen-Leng; Kranias, Evangelia G.; Akar, Fadi G.; Hajjar, Roger J.; Stillitano, Francesca; Pediatrics, School of MedicineAims: A mutation in the phospholamban (PLN) gene, leading to deletion of Arg14 (R14del), has been associated with malignant arrhythmias and ventricular dilation. Identifying pre-symptomatic carriers with vulnerable myocardium is crucial because arrhythmia can result in sudden cardiac death, especially in young adults with PLN-R14del mutation. This study aimed at assessing the efficiency and efficacy of in vivo genome editing, using CRISPR/Cas9 and a cardiotropic adeno-associated virus-9 (AAV9), in improving cardiac function in young adult mice expressing the human PLN-R14del. Methods and results: Humanized mice were generated expressing human wild-type (hPLN-WT) or mutant (hPLN-R14del) PLN in the heterozygous state, mimicking human carriers. Cardiac magnetic resonance imaging at 12 weeks of age showed bi-ventricular dilation and increased stroke volume in mutant vs. WT mice, with no deficit in ejection fraction or cardiac output. Challenge of ex vivo hearts with isoproterenol and rapid pacing unmasked higher propensity for sustained ventricular tachycardia (VT) in hPLN-R14del relative to hPLN-WT. Specifically, the VT threshold was significantly reduced (20.3 ± 1.2 Hz in hPLN-R14del vs. 25.7 ± 1.3 Hz in WT, P < 0.01) reflecting higher arrhythmia burden. To inactivate the R14del allele, mice were tail-vein-injected with AAV9.CRISPR/Cas9/gRNA or AAV9 empty capsid (controls). CRISPR-Cas9 efficiency was evaluated by droplet digital polymerase chain reaction and NGS-based amplicon sequencing. In vivo gene editing significantly reduced end-diastolic and stroke volumes in hPLN-R14del CRISPR-treated mice compared to controls. Susceptibility to VT was also reduced, as the VT threshold was significantly increased relative to controls (30.9 ± 2.3 Hz vs. 21.3 ± 1.5 Hz; P < 0.01). Conclusions: This study is the first to show that disruption of hPLN-R14del allele by AAV9-CRISPR/Cas9 improves cardiac function and reduces VT susceptibility in humanized PLN-R14del mice, offering preclinical evidence for translatable approaches to therapeutically suppress the arrhythmogenic phenotype in human patients with PLN-R14del disease.Item A reference tissue atlas for the human kidney(American Association for the Advancement of Science, 2022) Hansen, Jens; Sealfon, Rachel; Menon, Rajasree; Eadon, Michael T.; Lake, Blue B.; Steck, Becky; Anjani, Kavya; Parikh, Samir; Sigdel, Tara K.; Zhang, Guanshi; Velickovic, Dusan; Barwinska, Daria; Alexandrov, Theodore; Dobi, Dejan; Rashmi, Priyanka; Otto, Edgar A.; Rivera, Miguel; Rose, Michael P.; Anderton, Christopher R.; Shapiro, John P.; Pamreddy, Annapurna; Winfree, Seth; Xiong, Yuguang; He, Yongqun; de Boer, Ian H.; Hodgin, Jeffrey B.; Barisoni, Laura; Naik, Abhijit S.; Sharma, Kumar; Sarwal, Minnie M.; Zhang, Kun; Himmelfarb, Jonathan; Rovin, Brad; El-Achkar, Tarek M.; Laszik, Zoltan; He, John Cijiang; Dagher, Pierre C.; Valerius, M. Todd; Jain, Sanjay; Satlin, Lisa M.; Troyanskaya, Olga G.; Kretzler, Matthias; Iyengar, Ravi; Azeloglu, Evren U.; Kidney Precision Medicine Project; Medicine, School of MedicineKidney Precision Medicine Project (KPMP) is building a spatially specified human kidney tissue atlas in health and disease with single-cell resolution. Here, we describe the construction of an integrated reference map of cells, pathways, and genes using unaffected regions of nephrectomy tissues and undiseased human biopsies from 56 adult subjects. We use single-cell/nucleus transcriptomics, subsegmental laser microdissection transcriptomics and proteomics, near-single-cell proteomics, 3D and CODEX imaging, and spatial metabolomics to hierarchically identify genes, pathways, and cells. Integrated data from these different technologies coherently identify cell types/subtypes within different nephron segments and the interstitium. These profiles describe cell-level functional organization of the kidney following its physiological functions and link cell subtypes to genes, proteins, metabolites, and pathways. They further show that messenger RNA levels along the nephron are congruent with the subsegmental physiological activity. This reference atlas provides a framework for the classification of kidney disease when multiple molecular mechanisms underlie convergent clinical phenotypes.