Browsing by Author "Guo, Gordon Zhuo"

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    MiR-770-5p inhibits cisplatin chemoresistance in human ovarian cancer by targeting ERCC2
    (Impact Journals, 2016-08-16) Zhao, Henan; Yu, Xiaotang; Ding, Yanfang; Zhao, Jinyao; Wang, Guang; Wu, Xian; Jiang, Jiyong; Peng, Chun; Guo, Gordon Zhuo; Cui, Shiying; Department of Radiation Oncology, IU School of Medicine
    In this study, we examined the role of the miRNA miR-770-5p in cisplatin chemotherapy resistance in ovarian cancer (OVC) patients. miR-770-5p expression was reduced in platinum-resistant patients. Using a 6.128-fold in expression as the cutoff value, miR-770-5p expression served as a prognostic biomarker and predicted the response to cisplatin treatment and survival among OVC patients. Overexpression of miR-770-5p in vitro reduced survival in chemoresistant cell lines after cisplatin treatment. ERCC2, a target gene of miR-770-5p that participates in the NER system, was negatively regulated by miR-770-5p. siRNA-mediated silencing of ERCC2 reversed the inhibition of apoptosis resulting from miR-770-5p downreglation in A2780S cells. A comet assay confirmed that this restoration of cisplatin chemosensitivity was due to the inhibition of DNA repair. These findings suggest that endogenous miR-770-5p may function as an anti-oncogene and promote chemosensitivity in OVC, at least in part by downregulating ERCC2. miR-770-5p may therefore be a useful biomarker for predicting chemosensitivity to cisplatin in OVC patients and improve the selection of effective, more personalized, treatment strategies.