Browsing by Author "Ehrlich, Y."

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    Bioactive nanofibrous scaffolds for regenerative endodontics
    (SAGE, 2013-11) Bottino, M.C.; Kamocki, K.; Yassen, G.H.; Platt, J.A.; Vail, M.M.; Ehrlich, Y.; Spolnik, K.J.; Gregory, R.L.; Endodontics, School of Dentistry
    Here we report the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel antibiotic-containing scaffolds. Metronidazole (MET) or Ciprofloxacin/(CIP) was mixed with a polydioxanone (PDS)polymer solution at 5 and 25 wt% and processed into fibers. PDS fibers served as a control. Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), tensile testing, and high-performance liquid chromatography (HPLC) were used to assess fiber morphology, chemical structure, mechanical properties, and drug release, respectively. Antimicrobial properties were evaluated against those of Porphyromonas gingivalis/Pg and Enterococcus faecalis/Ef. Cytotoxicity was assessed in human dental pulp stem cells (hDPSCs). Statistics were performed, and significance was set at the 5% level. SEM imaging revealed a submicron fiber diameter. FTIR confirmed antibiotic incorporation. The tensile values of hydrated 25 wt% CIP scaffold were significantly lower than those of all other groups. Analysis of HPLC data confirmed gradual, sustained drug release from the scaffolds over 48 hrs. CIP-containing scaffolds significantly (p < .00001) inhibited biofilm growth of both bacteria. Conversely, MET-containing scaffolds inhibited only Pg growth. Agar diffusion confirmed the antimicrobial properties against specific bacteria for the antibiotic-containing scaffolds. Only the 25 wt% CIP-containing scaffolds were cytotoxic. Collectively, this study suggests that polymer-based antibiotic-containing electrospun scaffolds could function as a biologically safe antimicrobial drug delivery system for regenerative endodontics.
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    Endodontic Obturation: A Volumetric Analysis Using 3D Imaging Technology
    (Office of the Vice Chancellor for Research, 2015-04-17) Daetwyler, M.; Riad, K.; Ehrlich, Y.; Ghoneima, A.
    Abstract: A Root Canal (RC) obturation that fully occupies that prepared canal space contributes to treatment success. Invasive methods that involve sectioning of treated teeth have been used evaluate the quality of RC obturation. The aim of this study was to measure the amount of filling material in RC treated teeth using imaging methods: Cone Beam Computed tomography (CBCT) and 3D volumetric imaging software. These methods will be applied in an analysis of both Warm Vertical (WV) and Cold Lateral (CL) obturation methods. This noninvasive approach may be a preliminary step for future in vivo research. A noninvasive 3D imaging method was able to measure volume of RC fillings. Canals filled using WV contained a greater percentage of empty space than canals filled using the CL method in this study. This method could prove to be an efficient, cost effective tool for evaluating obturation material.