Browsing by Author "Cox, Cheryl"

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    Manufacturing Dendritic Cells for Immunotherapy: Monocyte Enrichment
    (Elsevier, 2020-01-15) Hopewell, Emily L.; Cox, Cheryl; Medical and Molecular Genetics, School of Medicine
    Dendritic cells play a key role in activation of the immune system as potent antigen-presenting cells. This pivotal position, along with the ability to generate dendritic cells from monocytes and ready uptake of antigen, makes them an intriguing vehicle for immunotherapy for a variety of indications. Since the first reported trial using dendritic cells in 1995, they have been used in trials all over the world for a plethora of indications. Monocyte-derived dendritic cells are generated from whole blood or apheresis products by culturing enriched monocytes in the presence of interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF). A variety of methods can be used for enrichment of monocytes for generation of clinical-grade dendritic cells and are summarized herein.
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    Tumor-infiltrating lymphocytes: Streamlining a complex manufacturing process
    (Elsevier, 2019-03) Hopewell, Emily L.; Cox, Cheryl; Pilon-Thomas, Shari; Kelley, Linda L.; Medical and Molecular Genetics, School of Medicine
    Adoptive cell therapy of tumor-infiltrating lymphocytes has shown promise for treatment of refractory melanoma and other solid malignancies; however, challenges to manufacturing have limited its widespread use. Traditional manufacturing efforts were lengthy, cumbersome and used open culture systems. We describe changes in testing and manufacturing that decreased the process cycle time, enhanced the robustness of critical quality attribute testing and facilitated a functionally closed system. These changes have enabled export of the manufacturing process to support multi-center clinical trials.