Browsing by Author "Chun, Kristin"

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    The proto‐oncogene function of Mdm2 in bone
    (Wiley, 2018-11) Olivos, David J., III; Perrien, Daniel S.; Hooker, Adam; Cheng, Ying-Hua; Fuchs, Robyn K.; Hong, Jung Min; Bruzzaniti, Angela; Chun, Kristin; Eischen, Christine M.; Kacena, Melissa A.; Mayo, Lindsey D.; Pediatrics, School of Medicine
    Mouse double minute 2 (Mdm2) is a multifaceted oncoprotein that is highly regulated with distinct domains capable of cellular transformation. Loss of Mdm2 is embryonically lethal, making it difficult to study in a mouse model without additional genetic alterations. Global overexpression through increased Mdm2 gene copy number (Mdm2Tg) results in the development of hematopoietic neoplasms and sarcomas in adult animals. In these mice, we found an increase in osteoblastogenesis, differentiation, and a high bone mass phenotype. Since it was difficult to discern the cell lineage that generated this phenotype, we generated osteoblast‐specific Mdm2 overexpressing (Mdm2TgOb) mice in 2 different strains, C57BL/6 and DBA. These mice did not develop malignancies; however, these animals and the MG63 human osteosarcoma cell line with high levels of Mdm2 showed an increase in bone mineralization. Importantly, overexpression of Mdm2 corrected age‐related bone loss in mice, providing a role for the proto‐oncogenic activity of Mdm2 in bone health of adult animals.
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    Setd1 Histone 3 Lysine 4 Methyltransferase Complex Components in Epigenetic Regulation
    (2011-03-16) Pick-Franke, Patricia A.; Skalnik, David Gordon; Chun, Kristin; Rhodes, Simon J.
    Setd1 histone 3 lysine 4 methyltransferases are critical for epigenetic regulation and gene expression. Setd1a is multiprotein complex comprised of several critical subunits including wdr82, which is essential for embryonic development, and cfp1, critical for regulation of both activation and repression of transcriptional programs required in basic and developmental cellular processes.