Browsing by Author "Chaudhari, Advika"

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    A Randomized Control Trial of Ravulizumab for Treatment of Patients With COVID-19 Infection and Kidney Injury
    (Elsevier, 2022) Memon, Aliza A.; Ahmed, Hasban; Li, Yi; Wongboonsin, Janewit; Hundert, Joshua; Benoit, Stefanie; Chaudhari, Advika; Sher, Jawad; Ghimire, Pratima; Hopkins, Rebecca; Patel, Jay; Stegman, Melinda; Lim, Kenneth; Azzi, Jamil; Siedlecki, Andrew M.; Medicine, School of Medicine
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    Integrin α 5 Is Regulated by miR-218-5p in Endothelial Progenitor Cells
    (Wolters Kluwer, 2022) Liu, Jialing; Li, Yi; Lyu, Lingna; Xiao, Liang; Memon, Aliza A.; Yu, Xin; Halim, Arvin; Patel, Shivani; Osman, Abdikheyre; Yin, Wenqing; Jiang, Jie; Naini, Said; Lim, Kenneth; Zhang, Aifeng; Williams, Jonathan D.; Koester, Ruth; Qi, Kevin Z.; Fucci, Quynh-Anh; Ding, Lai; Chang, Steven; Patel, Ankit; Mori, Yutaro; Chaudhari, Advika; Bao, Aaron; Liu, Jia; Lu, Tzong-Shi; Siedlecki, Andrew; Medicine, School of Medicine
    Kidney endothelial cells are sensitive to hypoxic injury. This cell type expresses integrin α5 (ITGA5), which is essential to the Tie2 signaling cascade. The microRNA miR-218 is known to increase after hypoxia, but the microRNA’s role in regulating ITGA5 protein synthesis is unclear. In this study, the authors found that miR-218-5p specifically binds to ITGA5 mRNA in human kidney-derived endothelial progenitor cells (EPCs). In an animal model of ischemia/reperfusion injury, cells pretreated with an miR-218-5p mimic were delivered efficiently, whereas an animal model containing an miR-218-2 deletion specific to angioblasts resulted in kidney dysgenesis and impaired migration of mouse kidney-derived EPCs. Understanding the regulation of prominent signaling pathways in EPCs may inform optimization of therapeutic techniques for addressing kidney endothelial cell injury.