Browsing by Author "Carr, Katherine A."

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    QUALITY CONTROL FROM A SUBSET OF HUMAN SURGICAL TISSUE SPECIMENS FROM THE IU SIMON CANCER CENTER TISSUE PROCUREMENT AND DISTRIBUTION CORE COLLECTED IN 2009-2010: AN H&E AND RIN VALUE ASSESSMENT
    (Office of the Vice Chancellor for Research, 2011-04-08) Spencer, Cleandrea; Sandusky, George; Rodgers, Matt; Carr, Katherine A.; Mitchell, Colleen
    Quality control (QC) of human tissue specimens for research is critical for the development of new bio-markers and their ability to determine clinical trial outcomes. In this study, we evaluated sixty-nine samples for both RNA and histology quality control measures from the IU Simon Cancer Center Tissue Bank. The IU Simon Cancer Center Tissue Bank is a centralized tissue procurement resource established to collect high quality tissue for basic clinical and translational research, collecting approximately 550 clinical cases per year using an informed consent and HIPAA signed document. All tissues are collected and processed in liquid nitrogen within 30 minutes of removal. The tissue samples are sliced and diced into 100 to 150 mg sample size. Each sample is placed into individual 2ml cryovials. Two representative samples are placed in 10% neutral buffered formalin. Two investigators QC the slides by microscopy to evaluate the following: percent of tumor, percent of necrosis, percent of fibrosis/inflammation, and percent of normal adjacent tissue. RNA was extracted using the Purescript RNA isolation kit (Gentra). Fifty-four of sixty-nine cases passed both histology and RNA (RIN value) QC. Of the fifteen cases that did not pass our QC criteria, thirteen cases did not pass the histology QC due to lack of tumor content (below 50%) in the sample, while the remaining two cases failed the RNA QC. Seventy-eight percent of samples passed our QC measures. The results were consistent with the existing literature on tissue quality control in human surgical tissue specimens.
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    Racial disparities in primary ciliary dyskinesia: Defining the problem and potential solutions
    (Wiley, 2023) Carr, Katherine A.; O’Connor, Michael G.; Shapiro, Adam J.; Machogu, Evans M.; Medicine, School of Medicine
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    Report from the pandemic influenza project: expert panel meetings
    (2008-08-27T17:03:37Z) Gaffney, Margaret M.; Carr, Katherine A.
    The Indiana University Center for Bioethics convened expert panels for each of the four topics of the Technical Advisory Documents (TADs): altered standards of care, triage, vaccine and antiviral allocation, and healthcare workforce management. The goal of each meeting was to assess the relevance and applicability of the recommendations put forth by the TADs. Participants were encouraged to apply the seven ethical points and assess the feasibility of the recommendations by examining case studies intended to portray real world ethical dilemmas. Engagement from the public was critical for each panel to learn the full ethical and social implications of pandemic influenza preparedness and planning.