Browsing by Author "Broxmeyer, H.E."

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    15. Augmentation of homing of cord blood stem cells
    (Elsevier, 2005-11) Broxmeyer, H.E.; Christopherson, K.W.; Hangoc, G.; Campbell, T.B.; Medicine, School of Medicine
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    Combinations of antioxidants and/or of epigenetic enzyme inhibitors allow for enhanced collection of mouse bone marrow hematopoietic stem cells in ambient air
    (Elsevier, 2018-07) Cai, Q.; Capitano, M.; Huang, X.; Guo, B.; Cooper, S.; Broxmeyer, H.E.; Microbiology and Immunology, School of Medicine
    Hematopoietic cell transplantation (HCT) is a treatment for malignant and non-malignant disorders. However, sometimes the numbers of donor hematopoietic stem cells (HSC) are limiting, which can compromise the success of HCT. We recently published that collection and processing of mouse bone marrow (BM) and human cord blood cells in a hypoxic atmosphere of 3% O2 or in ambient air (~21% O2) in the presence of cyclosporine A yields increased numbers of HSC. We now show that collection and processing of mouse BM cells in ambient air in the presence of specific combinations of anti-oxidants and/or inhibitors of epigenetic enzymes can also enhance the collection of HSC, information of potential relevance for enhanced efficacy of HCT.
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    Insights into the biology of cord blood stem/progenitor cells
    (Wiley, 2011-04) Broxmeyer, H.E.; Microbiology and Immunology, School of Medicine
    Objectives:  To review information on cord blood banking and transplantation with respect to the author’s studies, and in context of this field of investigation. Results:  Cord blood transplantation has been successfully used to treat a number of malignant and non‐malignant disorders. However, this technique is still associated with limited numbers of cells for transplantation, and with delayed engraftment of neutrophils and platelets. The field of cord blood transplantation will benefit from enhanced and mechanistically based information on haematopoietic stem cell function and potential means to enhance its effectiveness are reviewed. This includes notions concerning possibility of retrieving more cells from the placenta and cord blood, to expand haematopoietic stem cells ex vivo and to increase efficiency of homing and engraftment of these cells. Also discussed are cryopreservation and long‐term storage of cord blood haematopoietic and progenitor cells, and new laboratory findings and animal studies for non‐haematopoietic uses of cord blood.