Browsing by Author "Batra, Chandni"

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    An in-vitro comparison of four antibacterial agents with and without nicotine and their effects on human gingival fibroblasts
    (Wiley, 2021) Batra, Chandni; Alalshaikh, Marwa; Gregory, Richard L.; Windsor, L. Jack; Blanchard, Steven B.; Hamada, Yusuke; Periodontology, School of Dentistry
    Background To compare anti-bacterial activity of 0.12% Chlorhexidine (CHX), 10% Povidone Iodine (PVD), Vega Oral Care Gel (VEGA), and Antioxidant Gel (AO) on Streptococcus mutans, Streptococcus sanguis, Fusobacterium nucleatum, and Porphyromonas gingivalis with and without nicotine and to evaluate their effects on human gingival fibroblasts (HGFs). Methods S. mutans, S. sanguis, P. gingivalis, and F. nucleatum were incubated with serial dilutions (1/4, 1/8, 1/16, 1/32, and 1/64) of anti-bacterial agents in media (with and without nicotine). Minimum inhibitory and minimum bactericidal concentrations (MIC/MBC) were measured, and confocal microscopy was performed. HGFs were exposed to serial dilutions (1/10, 1/100, 1/1000, and 1/10,000) of antibacterial agents with media. Water-soluble tetrazolium-1 (WST-1) assay and lactate dehydrogenase (LDH) assay were used to assess proliferation and cytotoxicity towards HGFs. Results CHX and PVD significantly inhibited growth of all bacterial species (P < 0.0001) at all dilutions. AO and VEGA inhibited growth of all bacterial species up to only the 1/4 dilution. CHX and PVD decreased HGF proliferation at 1/10 and 1/100 dilution, whereas AO at all dilutions (P < 0.05). CHX and AO were cytotoxic at all dilutions (P < 0.05). VEGA was not cytotoxic to HGFs and did not affect HGF proliferation at any dilution (P > 0.05). An increased bacterial growth was seen for all species except P. gingivalis with addition of nicotine. Conclusion CHX and PVD demonstrate superior antibacterial properties, but significantly reduce HGF proliferation. AO is bacteriostatic at lower dilutions but is highly toxic to HGFs. VEGA was bacteriostatic and demonstrated no detrimental effects on HGF's.
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    Role of Medication in Osseointegration of Dental Implants
    (2019-05) Ibraheem, Ahmed; Batra, Chandni; John, Vanchit; Shin, Daniel; Periodontology, School of Dentistry
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    The Effects of Two rhPDGF-BB Applications on Periodontal Ligament Cell’s Proliferation and VEGF Expression
    (2024) Velgis, Raziel; Windsor, L. Jack; John, Vanchit; Batra, Chadni; Batra, Chandni; John, Vanchit; Batra, Chandni; Windsor, Jack
    Background: Platelet derived growth factor (PDGF) has been shown since the late 1980s to play a major role in periodontal regeneration. PDGF has five isoforms and the isoform PDGF-BB has been found to be the most effective. Animal studies evaluating release kinetics of purified recombinant human platelet-derived growth factor (rh-PDGF-BB), demonstrated that 90% of rh-PDGF-BB was depleted from sites within 72 hours after implantation. Thus, the aim of this in-vitro study was to evaluate the effects of two rhPDGF-BB applications on periodontal ligament fibroblasts proliferation and VEGF expression. Materials & Methods: Periodontal ligament (PDL) cells were seeded in multiple 6 well plates and split into 3 groups. Group 1 served as a control that received no rh-PDGF-BB. Group 2 was given an initial dose of 10 ng/ml rhPDGF-BB on day 1 and none on day 3. Group 3 received a dose of 10 ng/ml rhPDGF-BB on day 1 and a second dose on day 3. On day 3, half of the plates were stopped and media collected was used for LDH, WST-1 and VEGF ELISA analysis for evaluation of VEGF expression, cellular proliferation and cytotoxicity. Plain media was then added to groups 1 and 2 while group 3 received media with rh-PDGF-BB. On day 6, the collected media was used for ELISA, WST-1 and LDH assays. Results: PDL cell proliferation was not significantly different between any group studied (p=0.689). VEGF expression was increased in group 2 and 3 on day 3 compared to group 1 (p=0.005). There was no difference between groups 1 and 2 on day 6 (p=0.977). Conclusion: The addition of rhPDGF-BB to PDL cells did not increase proliferation at any time point. A single application of rhPDGF-BB increased the expression of VEGF from PDL cells; however, an additional application did not significantly increase the expression of VEGF.