Browsing by Author "Aldrich, Carla J."

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    Anti-STAT6 CTL activity in Stat6−/− mice: A cautionary tale
    (Taylor & Francis, 2013) Kaplan, Mark H.; Cundiff, Judy K.; Smith, Jill Stader; Aldrich, Carla J.; Pediatrics, School of Medicine
    The generation of germline gene mutations in mice has been an invaluable tool for experimental biology. However, studying immune responses that develop in the absence of a specific protein that could alter thymic selection complicates experimental interpretations. We observed that CD8(+) T cells from Stat6 (-/-) mice displayed "autoreactivity" to STAT6-expressing cells, associated with specific STAT6 peptides binding to MHC class I molecules. These results suggest caution in interpreting experiments where STAT6-expressing cells are transferred into Stat6 (-/-) mice, or where adoptive transfer of Stat6 (-/-) lymphocytes is performed. Our results further highlight additional considerations when studying immune responses involving cell transfer into gene-deficient mice.