Browsing by Author "Al-Shibani, Nouf"

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    COVID-19 Pandemic Driven Knowledge, Attitude, Clinical Practice, Distress Reactions, and Post-Traumatic Growth of Dental Care Providers in Riyadh City, Saudi Arabia: A Cross-Sectional Study
    (2021) Labban, Nawaf; Alfouzan, Afnan F.; Al-Shibani, Nouf; Al-Otaibi, Hanan N.; Al Taweel, Sara Mohammad; Al Ghanem, Shahad Hamad; Schrader, Stuart M.; Biomedical Sciences and Comprehensive Care, School of Dentistry
    Objective: The present study aimed to assess knowledge, attitude, clinical practices, distress reactions, and post-traumatic growth of dentists during the COVID-19 pandemic in Riyadh City. Methodology: An online questionnaire was developed to assess various dental professionals from both governmental and private sectors during the early COVID-19 outbreak in Riyadh City, Saudi Arabia. The questionnaire was framed to assess the demographic variables, knowledge, attitude, and clinical practices towards handling the COVID-19 pandemic. Furthermore, the general anxiety disorder-7 (GAD-7), worry questionnaire items, short form of post-traumatic growth inventory (PTGI-SF), and life satisfaction questionnaire items were developed to assess the anxiety, worries, post-traumatic growth, and life satisfaction of dental care providers following the traumatic events. Data were analysed using SPSS 22.0 and R environment ver.3.2.2. Continuous measurement results were presented as mean ± SD, and categorical measurements results were presented in number (%). Results: Our study's significant findings revealed that a majority of the dentists were in the age group of 21-30 and practiced general dentistry. The study participants in age groups 51–60 and 41–50 years old showed more liability in perceiving COVID-19 as an extremely dangerous disease than 31–40 and 21–30 age groups. The majority of respondents (63.4%) had an anxiety scale score of greater than 40, followed by 22.8% with scores 20-40 and 13.9% with scores less than 20, respectively. The mean scores for the GAD-7 and the worry items were 8.24 ± 3.21 and 13.92 ± 4.77, respectively. The data showed that most survey participants had adopted moderate post-traumatic changes during the pandemic, as evident by their mean score of 34.17 ± 3.40. The life satisfaction of the participants was neutral, as demonstrated by their mean score of 20.16 ± 4.03. Conclusion: This study’s findings delineated that the COVID-19 pandemic situation has influenced dentists' mental health, with a moderate level of anxiety, and worries among other psychological symptoms. Furthermore, there was adequate knowledge regarding the COVID-19 among dentists; however, the pandemic affected the financial conditions of the respondents. There was a moderate level of post-traumatic growth and neutral life satisfaction of the dental providers.
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    THE EFFECTS OF TOBACCO TREATED PORPHYROMONAS GINGIVALIS ON HUMAN EPITHELIAL CELLS
    (Office of the Vice Chancellor for Research, 2012-04-13) Tursunova, Roziya H.; Al-Shibani, Nouf; Windsor, L. Jack; Gregory, Richard L.
    Bacteria and tobacco are risk factors for periodontal diseases. Bacteria-host interactions play a critical role in disease development and progression. The effects of tobacco-treated bacteria such as Porphyromonas gingivalis on epithelial cells have not yet been examined. Therefore, P. gingivalis were treated with different tobacco products (nicotine, cigarette smoke conden-sate (CSC), and dissolvable smokeless tobacco (DST) strips) to determine the effects that they have on epithelial cells. P. gingivalis were grown with or without the products for 24 hours at 37◦C. The cells were separated from the supernatant, washed with 0.9% NaCl and incubated at 60◦C to kill the bacte-ria. Protein assays was performed to determine the protein concentration in the cell pellets and supernatants. Lactate dehydrogenase (LDH) assays are being used to measure the cytotoxicity of the cells and supernatants on epi-thelial cells in a dose dependent manner. Non-toxic amounts of the cell pel-lets and supernatants will be used to treat epithelial cells for 72 hours and the media analyzed by cytokine/growth factor protein arrays. The protein assays showed that CSC and nicotine treated P. gingivalis cells had less pro-tein than the others. The total protein in the supernatant for the CSC treated bacteria was less compared to others. The protein data suggests that CSC and nicotine affect protein expression in and by the P. gingivalis cells. To-bacco-treated bacteria are hypothesized to increase the expression of pro-inflammatory cytokines/growth factors by the epithelial cells, thereby con-tributing to the inflammation seen in periodontal diseases. This research was funded by Indiana University-Purdue University Indianapolis, Multidisci-plinary Undergraduate Research Institute (MURI).