2D and 3D In Vitro Co-culture for Cancer and Bone Cell Interaction Studies
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2019
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American English
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Springer Nature
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Abstract
Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.
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Cite As
Marino S, Bishop RT, de Ridder D, Delgado-Calle J, Reagan MR. 2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies. Methods Mol Biol. 2019;1914:71-98. doi:10.1007/978-1-4939-8997-3_5
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Methods in Molecular Biology
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PMC
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Article
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Author's manuscript