Browsing by Subject "Lipid nanoparticles"

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    Preparation and Characterization of IL-22 mRNA-loaded Lipid Nanoparticles
    (Bio-Protocol, 2023-04-05) Alghoul, Zahra; Sung, Junsik; Wu, Kenji; Alpini, Gianfranco; Glaser, Shannon; Yang, Chunhua; Merlin, Didier; Medicine, School of Medicine
    Interleukin-22 (IL-22) has been demonstrated as a critical regulator of epithelial homeostasis and repair; it showed an anti-inflammatory effect against ulcerative colitis. Local microinjection of IL-22 cDNA vector has been shown to be effective in treating ulcerative colitis in mouse models. However, microinjection comes with multiple technical challenges for routine colon-targeted drug delivery. In contrast, oral administration can get around these challenges and provide comparable efficacy. We showed in previous studies that oral administration of new lipid nanoparticles (nLNP)-encapsulated IL-22 mRNA targets the colon region and efficiently ameliorates colitis. This protocol describes the details of preparing and characterizing the nLNP-encapsulated IL-22 mRNA using three major lipids that mimic the natural ginger-derived nanoparticles. It provides an nLNP platform that can be used to orally deliver other types of nucleic acids to the colon.