Browsing by Subject "Auditory hair cells"

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    Large-scale annotated dataset for cochlear hair cell detection and classification
    (Springer Nature, 2024-04-23) Buswinka, Christopher J.; Rosenberg, David B.; Simikyan, Rubina G.; Osgood, Richard T.; Fernandez, Katharine; Nitta, Hidetomi; Hayashi, Yushi; Liberman, Leslie W.; Nguyen, Emily; Yildiz, Erdem; Kim, Jinkyung; Jarysta, Amandine; Renauld, Justine; Wesson, Ella; Wang, Haobing; Thapa, Punam; Bordiga, Pierrick; McMurtry, Noah; Llamas, Juan; Kitcher, Siân R.; López-Porras, Ana I.; Cui, Runjia; Behnammanesh, Ghazaleh; Bird, Jonathan E.; Ballesteros, Angela; Vélez-Ortega, A. Catalina; Edge, Albert S. B.; Deans, Michael R.; Gnedeva, Ksenia; Shrestha, Brikha R.; Manor, Uri; Zhao, Bo; Ricci, Anthony J.; Tarchini, Basile; Basch, Martín L.; Stepanyan, Ruben; Landegger, Lukas D.; Rutherford, Mark A.; Liberman, M. Charles; Walters, Bradley J.; Kros, Corné J.; Richardson, Guy P.; Cunningham, Lisa L.; Indzhykulian, Artur A.; Otolaryngology -- Head and Neck Surgery, School of Medicine
    Our sense of hearing is mediated by cochlear hair cells, of which there are two types organized in one row of inner hair cells and three rows of outer hair cells. Each cochlea contains 5-15 thousand terminally differentiated hair cells, and their survival is essential for hearing as they do not regenerate after insult. It is often desirable in hearing research to quantify the number of hair cells within cochlear samples, in both pathological conditions, and in response to treatment. Machine learning can be used to automate the quantification process but requires a vast and diverse dataset for effective training. In this study, we present a large collection of annotated cochlear hair-cell datasets, labeled with commonly used hair-cell markers and imaged using various fluorescence microscopy techniques. The collection includes samples from mouse, rat, guinea pig, pig, primate, and human cochlear tissue, from normal conditions and following in-vivo and in-vitro ototoxic drug application. The dataset includes over 107,000 hair cells which have been identified and annotated as either inner or outer hair cells. This dataset is the result of a collaborative effort from multiple laboratories and has been carefully curated to represent a variety of imaging techniques. With suggested usage parameters and a well-described annotation procedure, this collection can facilitate the development of generalizable cochlear hair-cell detection models or serve as a starting point for fine-tuning models for other analysis tasks. By providing this dataset, we aim to give other hearing research groups the opportunity to develop their own tools with which to analyze cochlear imaging data more fully, accurately, and with greater ease.
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    TMIE defines pore and gating properties of the mechanotransduction channel of mammalian cochlear hair cells
    (Cell Press, 2020-07-08) Cunningham, Christopher L.; Qiu, Xufeng; Wu, Zizhen; Zhao, Bo; Peng, Guihong; Kim, Ye-Hyun; Lauer, Amanda; Müller, Ulrich; Otolaryngology -- Head and Neck Surgery, School of Medicine
    TMC1 and TMC2 (TMC1/2) have been proposed to form the pore of the mechanotransduction channel of cochlear hair cells. Here, we show that TMC1/2 cannot form mechanotransduction channels in cochlear hair cells without TMIE. TMIE binds to TMC1/2, and a TMIE mutation that perturbs TMC1/2 binding abolishes mechanotransduction. N-terminal TMIE deletions affect the response of the mechanotransduction channel to mechanical force. Similar to mechanically gated TREK channels, the C-terminal cytoplasmic TMIE domain contains charged amino acids that mediate binding to phospholipids, including PIP2. TMIE point mutations in the C terminus that are linked to deafness disrupt phospholipid binding, sensitize the channel to PIP2 depletion from hair cells, and alter the channel's unitary conductance and ion selectivity. We conclude that TMIE is a subunit of the cochlear mechanotransduction channel and that channel function is regulated by a phospholipid-sensing domain in TMIE with similarity to those in other mechanically gated ion channels.