Browsing by Author "Wooden, William"

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    Distinct Effects of Adipose-derived Stem Cells and Adipocytes on Normal and Cancer Cell Hierarchy
    (AACR, 2016-07) Anjanappa, Manjushree; Burnett, Riesa; Zieger, Michael A.; Merfeld-Clauss, Stephanie; Wooden, William; March, Kieth; Tholpady, Sunil; Nakshatri, Harikrishna; Department of Surgery, IU School of Medicine
    Adipose-derived stem cells (ASC) have received considerable attention in oncology because of the known direct link between obesity and cancer as well as the use of ASCs in reconstructive surgery after tumor ablation. Previous studies have documented how cancer cells commandeer ASCs to support their survival by altering extracellular matrix composition and stiffness, migration, and metastasis. This study focused on delineating the effects of ASCs and adipocytes on the self-renewal of stem/progenitor cells and hierarchy of breast epithelial cells. The immortalized breast epithelial cell line MCF10A, ductal carcinoma in situ (DCIS) cell lines MCF10DCIS.com and SUM225, and MCF10A-overexpressing SRC oncogene were examined using a mammosphere assay and flow cytometry for the effects of ASCs on their self-renewal and stem-luminal progenitor-differentiated cell surface marker profiles. Interestingly, ASCs promoted the self-renewal of all cell types except SUM225. ASC coculture or treatment with ASC conditioned media altered the number of CD49fhigh/EpCAMlow basal/stem-like and CD49fmedium/EpCAMmedium luminal progenitor cells. Among multiple factors secreted by ASCs, IFNγ and hepatocyte growth factor (HGF) displayed unique actions on epithelial cell hierarchy. IFNγ increased stem/progenitor-like cells while simultaneously reducing the size of mammospheres, whereas HGF increased the size of mammospheres with an accompanying increase in luminal progenitor cells. ASCs expressed higher levels of HGF, whereas adipocytes expressed higher levels of IFNγ. As luminal progenitor cells are believed to be prone for transformation, IFNγ and HGF expression status of ASCs may influence susceptibility for developing breast cancer as well as on outcomes of autologous fat transplantation on residual/dormant tumor cells. Implications: This study suggests that the ratio of ASCs to adipocytes influences cancer cell hierarchy, which may impact incidence and progression.
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    Novel Preoperative Patient-centered Surgical Wellness Program Impacts Length of Stay Following Pancreatectomy
    (International Institute of Anticancer Research, 2021-04) Soufi, Mazhar; Deperalta, Danielle K.; Simpson, Rachel; Flick, Katelyn; Yip-Schneider, Michele T.; Schmidt, Christian M., II; Kilbane, Molly; Colgate, Cameron; Kelley, Kristen E.; Wooden, William; Ceppa, Eugene P.; House, Michael; Zyromski, Nicholas; Nakeeb, Atilla; Schmidt, C. Max; Surgery, School of Medicine
    Background/Aim: We created a novel, preoperative wellness program (WP) that promotes recovery. This study assessed its impact on patient outcomes after pancreatectomy. Patients and Methods: Pancreatoduodenectomies (PD) and distal pancreatectomies (DP) performed from 2015 to 2018 were reviewed using our institutional NSQIP database. Patients in the WP had their medical conditions optimized and were provided with the following: chlorhexidine, topical mupirocin, incentive spirometer, and immune-nutrition supplements. Results: Out of a total of 669 pancreatectomy patients (411 PD, 258 DP), 308 were enrolled in the WP (188 PD, 120 DP). In the PD subgroup, on multivariable analysis (MVA), the WP patients had shorter lengths of hospital stay (LOS) (12 vs. 10 days, p<0.001). On MVA, WP patients had less post-op transfusion (20 vs. 10%, p=0.027). For the combined groups on MVA, LOS continued to be significant (OR=0.89, 95%CI=0.82-0.97, p<0.007). Conclusion: A preoperative patient centered WP may reduce the length of stay.
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    Recurrent Melanoma In Situ at the Skin Graft Site of a Prior Malignant Melanoma of the Left Upper Extremity: A Case Report
    (Springer Nature, 2025-05-31) Toliver, Brandon C.; Johnson, Megan; Wooden, William; Dermatology, School of Medicine
    We present the case of a 43-year-old Caucasian, fair-skinned male with a history of multiple malignant melanomas, who presented in February 2024 for excision of a new melanoma arising within a prior skin graft. The graft had been harvested from his thigh to close an excision of a primary malignant melanoma with a depth of 0.73 mm in 2018. Extensive pathologic interrogation, as well as systemic work-up - including positron emission tomography and computed tomography scans - was performed to determine whether this lesion represented local recurrent disease or a new primary melanoma. This workup was consistent with a primary melanoma in situ, which led to the decision to proceed with skin graft resection and full-thickness skin grafting from his lower left abdomen. The entire previous skin graft was excised. Subsequent surgical pathologic examination of the excised skin graft demonstrated no residual malignant melanoma. This interesting case demonstrates a unique site of primary melanoma occurrence in a split-thickness skin graft. While this may be the result of incomplete tumor resection at the primary operation, the initial excision had wide margins of 2 cm, and pathological examination demonstrated negative margins. Additionally, the skin graft harvest site was carefully selected, as it came from a relatively sun-protected site. This presentation highlights the importance of continued surveillance of melanoma excision sites, warning patients of possible tumor recurrence or new primary occurrence, and setting expectations that incidental findings may occur.