Yan, LiboXu, Yan2017-06-062017-06-062015-08-20Yan, L., & Xu, Y. (2015). Adoptive Transfer of Myeloid-Derived Suppressor Cells and T Cells in a Prostate Cancer Model. Bio-Protocol, 5(16), e1557.https://hdl.handle.net/1805/12865The adoptive transfer of immune cells for cancer, chronic infection, and autoimmunity is an emerging field that has shown promise in recent trials. The transgenic adenocarcinoma mouse prostate (TRAMP) is a classical mouse model of prostate cancer (PCa) and TRAMP cell lines were derived from a TRAMP mouse tumor. TRAMP-C2 is tumorigenic when subcutaneously (s.c.) grafted into syngeneic C57BL/6 host mice (Foster et al., 1997). This protocol will describe the adoptive transfer of purified CD11b(+)Gr1(+) double positive (DP) myeloid-derived suppressor cells (MDSC) and CD3(+) T cells in the TRAMP-C2 prostate cancer mouse model in order to establish the intrinsic functionality of these immune cells and to determine their role in tumorigenesis in vivo (Yan et al., 2014).en-USPublisher PolicyCell transfer therapyimmunologyProstate CancerArticle